端粒酶启动子肿瘤靶向肿瘤坏死因子相关凋亡诱导配体基因抗人肠癌细胞的活性研究  

Activity of the TNF-related Apoptosis-inducing Ligand(TRAIL)gene on colon cancer line expressed from the hTERT promoter.

作　　者：陈斌[1] 何超[2] 劳伟峰[2] 黄学锋[2] 方炳良[3] 

机构地区：[1]温州医学院附属台州医院普外科,浙江台州317000 [2]浙江大学医学院附属邵逸夫医院肿瘤科,浙江杭州710016 [3]美国MD.Anderson癌症中心

出　　处：《温州医学院学报》2005年第5期349-352,共4页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30271467) 。

摘　　要：目的：探讨端粒酶(hTERT)启动子驱动下肿瘤坏死因子相关凋亡诱导配体(TNF-related Apoptosis-inducing Ligand，TRAIL)基因在大肠癌细胞 DLD1 和 HT-29的表达及其杀细胞作用。方法：通 过腺病毒载体系统将 hTERT 启动子驱动的 TRAIL 基因转入大肠癌细胞 DLD1和 HT-29，流式细胞仪检测 GFP/ TRAIL(绿色荧光蛋白和 TRAIL 融合基因)的表达和细胞凋亡率，MTT 法检测细胞生长抑制率。结果：端粒 酶启动子驱动的 GFP/TRAIL 基因和 CMV 启动子驱动的 GFP(绿色荧光蛋白)基因在 DLD1和 HT-29内的表 达平分别达41． 63%和31． 4%；GFP/TRAIL 基因对 DLD1和 HT-29细胞的生长抑制率分别达93． 5%和74． 2%， 凋亡率分别是41． 1%和25． 8%，与 PBS 和 Ad/CMV-GFP 的差异都有显著意义(P＜0． 05) 。结论：端粒酶启 动子驱动的 GFP/TRAIL 融合基因在大肠癌细胞 DLD1和 HT-29中能够有效的表达；TRAIL 基因对大肠癌细 胞 DLD1和 HT-29有明显的抑制生长和促凋亡作用。Objective：To evaluate the expression and activity of GFP/TRAIL gene on colon cancer line DLD1 and HT-29 expressed from the hTERT promoter. Me thods：GFP/TRAIL gene expressed from the hTERT promoter was transfected into DLD-1 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein was determined under Fluorescence-activated cell sorting （FACS）. the effects of the GFP/TRAIL gene on the inhibition of cell growth were also determined by MTT assay. Results：The expression of GFP gene was 41.63% and 31.4% with either hTERT promoter in DLD1 and HT-29 cells, respectively; GFP/TRAIL gene was able to inhibit cell growth（93.5% and 74.2%） and induce apoptosis（41.1% and 25.8%）of DLD-1 and HT-29, respectively, there was significant difference between Ad/hTERT-gTRAIL and the other two control group （PBS and Ad/CMV^FP）（ P〈 0.05）; Conclusion^The expression of GFP/TRAIL gene from the hTERT promoter by adenoviral vector system is obvious in DLD-1 and HT-29 cell： GFP/TRAIL gene expression can both inhibit cell growth and induce apoptosis of colon cancer cell line DLD-1 and HT-29.

关 键 词：TRAIL基因 结肠肿瘤 基因治疗 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]