一株水禽流感病毒分离株表面膜蛋白基因的序列分析  

Molecular Analysis of the Surface Glycoprotein Genes of an Aquatic Bird Influenza Virus

作　　者：张评浒[1] 薛峰[1] 唐应华[1] 钱忠明[1] 郝贵杰[1] 刘慧谋[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《中国病毒学》2005年第5期555-557,共3页Virologica Sinica

基　　金：国家科技攻关计划项目(2004BA519A02);江苏省属高校重大基础研究项目(05KJA23016)

摘　　要：Several H11N2 subtype of Avian influenza A viruses were isolated from aquatic birds in live bird markets when we surveyed the ecology of the influenza in East China for more than two years and identified by specific RT-PCR.The hemagglutinin(HA) and neuraminidase(NA) genes of one representative virus named A/Duck/Yangzhou/44/2002(H11N2)(DYZ/44/02) was sequenced.The results showed the HA nucleotide sequence of DYZ/44/02 has high identity with Dk/England/56(H11N6) and the NA nucleotide sequence of DYZ/44/02 has more than 95% sequence homology with Dk/Hokkaido/49/98(H9N2).Sequencing and phylogenetic analysis of the N2 NA genes of DYZ/44/02 revealed that the NA gene of DYZ/44/02 has close relationships with that of Ck/Korea/MS96/96-like H9N2 virus and are distinct from those of Ck/Beijing/94(H9N2).The sequence of cleavage site of DYZ/44/02 consists of a single arginine,as is the case with most other hemagglutinins exhibiting low susceptibility to proteolytic activation.Several H11N2 subtype of Avian influenza A viruses were isolated from aquatic birds in live bird markets when we surveyed the ecology of the influenza in East China for more than.two years and identified by specific RT-PCR. The hemagglutinin （HA） and neuraminidase （NA） genes of one representative virus named A/Duck/Yangzhou/44/2002 （H11N2） （DYZ/44/02） was sequenced. The results showed the HA nucleotide sequence of DYZ/44/02 has high identity with Dk/England/56 （H11N6） and the NA nucleotide sequence of DYZ/44/02 has more than 95% sequence homology with Dk/Hokkaido/49/98 （H9N2）. Sequencing and phylogenetic analysis of the N2 NA genes of DYZ/44/02 revealed that the NA gene of DYZ/44/02 has close relationships with that of Ck/Korea/MS96/96-1ike H9N2 virus and are distinct from those of Ck/Beijing/94 （H9N2）. The sequence of cleavage site of DYZ/44/02 consists of a single arginine, as is the case with most other hemagglutinins exhibiting low susceptibility to proteolytic activation.

关 键 词：禽流感病毒 H11N2亚型 HA NA 剪切位点 

分 类 号：S852.65[农业科学—基础兽医学]