gax和HIF-1α基因在原发性肝癌组织中的表达及意义  

Expression of hypoxia inducible factor-1α and homeobox gene gax in primary hepatocellular carcinoma

作　　者：杨镛[1] 段志泉[1] 辛世杰[1] 陈翠菊[2] 罗开元[3] 时德[4] 

机构地区：[1]中国医科大学附属第一医院普外三科,辽宁沈阳110001 [2]成都军区昆明总医院血管外科暨成都军区血管外科中心,云南昆明650032 [3]云南省第二人民医院普外科,云南昆明650021 [4]重庆医科大学附属第一医院普外二科,重庆400016

出　　处：《中国现代普通外科进展》2005年第1期14-16,共3页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金资助项目(30000163)

摘　　要：目的探讨同源盒基因(gax)和缺氧诱导因子(HIF1α)表达产物与原发性肝癌发生、发展的关系。方法采用逆转录聚合酶链反应(RTPCR)和蛋白质印迹技术(Westernblot)分别检测20例原发性肝癌患者癌及癌旁肝组织中gax和HIF1α的mRNA以及蛋白质表达水平。结果肝癌组织中HIF1αmRNA(118.1±33.2)及蛋白表达(10.86±2.76)水平显著高于癌旁组织(分别为42.7±10.5与3.52±1.53,P均<0.05);肝癌组织中gaxmRNA表达(39.5±13.8)及其蛋白表达(4.10±1.26)水平低于癌旁组织(P均<0.05)。结论gax和HIF1α基因是调控肝癌组织血管生长的重要基因之一,与gax表达下调及HIF1α表达下调肝癌发生发展密切相关。Objcetive：To explore the expression of hyPoxia Inducible faator-1α（HIF-1α）and homeobox gene Gax in hepatocellular carclnoma（HCC）, and to evaluate the relationship between HIF-1α and gax and between HCC.Methods ：Reverse transcription polymerase chain reaction （RT-PCR）and protein electrophoresis technique were used to detect the expression level of HIF- 1α and gax in surgically resected hepatocellular carcinoma and adjacent non-cancerous tissues in 20 cases. Results .The expression level of HIF-1α and gax were clearly observed In hepatic tissues from 20 patients. At the protein and mRNA levels,the expression ration of HIF-1α in hepatocellular carcinoma was increased significantly compared with those in para-oarcinoma tissues. There was remarkable difference in expression of HIF-1α between the hepatooellular carcinoma tissues（118.1 ±33.2 or 10.86±2.76）and the adjacent non-cancerous tissues（42.7 ±10.5 or 3.52 ±1.53）,P〈0.05. And it was significante difference in the expression of gax between the hepatocellular carcinoma tissues（39.5± 13.8 or 4.10 ± 1. 26）and the para-carcinoma tissues（50.7± 16.8 or 6.65 ± 2.25）, P〈 0. 05. Con clusloa：ln the procedure of growth on the hepatocellular carcinoma tissue,HIF-1α and gax genes play a crucial role ,which may be one of important genes for canceration of liver cells.

关 键 词：肝肿瘤 缺氧诱导因子-1Α 同源盒基因 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]