反义寡核苷酸对胆囊癌细胞端粒酶活性作用的实验研究(英文)  

Antisense oligonucleotide mediated inhibition on telomerase activity in gallbladder carcinoma cell

作　　者：靳斌 姜希宏 王伟 徐克森 史朝晖 

机构地区：[1]Department of General Surgery,Qilu Hospital of Shandong University,Jinan 250012,China [2]Department of Molecular Biology Experiment Center,School of Medicine,Shandong University,Jinan 250012,China

出　　处：《中国现代普通外科进展》2005年第1期27-30,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的探讨反义寡核苷酸对胆囊癌细胞端粒酶活性的影响及对胆囊癌细胞生长增殖的作用。方法设计针对端粒酶RNA亚基模板序列并经硫代磷酸修饰的反义、正义和随机序列寡核苷酸,并以正常人成纤维细胞作对照,观察其对人胆囊癌细胞(GBCSD)端粒酶活性和细胞生长增殖的影响作用以及对正常人成纤维细胞的作用。结果反义寡核苷酸可有效地抑制胆囊癌细胞的端粒酶活性,呈剂量依赖性,并明显的抑制胆囊癌细胞的生长,对正常人成纤维细胞生长无明显作用。结论硫代反义寡核苷酸(PSODN)对胆囊癌端粒酶活性有明显的抑制用并促进细胞凋亡。Objective ： TO study the antisense oligonucleotide mediated inhibition on telomerase activity and cell proliferation of GBC-SD cell. Methods ：We design the antisense,sense, and random oligonucleotide with phosphoric acid modification for the hTR（Human Telomerase RNA） template sequence. MTT and PCR methods were used to observe the inhibition on telomerase activity and cell proliferation of GBC-SD cell ,and fibroblast cells were used as control group. Results ：PS-ODN can lead to the reduction of cell survival rate of GBC-SD cell, wich dosage dependence. The experimental group cell detected by scanning electron appeared apoptotic feature. Conclusion ：PS-ODN can inhibit telomerase activity of GBC-SD cell effectively and induce the cell apoptosis.

关 键 词：胆囊肿瘤 端粒酶 寡核苷酸类 反义 

分 类 号：R735.8[医药卫生—肿瘤] Q78[医药卫生—临床医学]