P物质-PLGA纳米缓释微粒的制备及其性质测定  

Preparation of subsance P-loaded PLGA nanoparticles and determination of their properties

作　　者：汪银雄[1] 段银钟[1] 吴红[2] 孙应明 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032 [2]第四军医大学药物化学教研室 [3]四川大学华西口腔医学院

出　　处：《临床口腔医学杂志》2005年第11期657-659,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助(30200320)

摘　　要：目的:制备P物质(Substance P,SP)PLGA(poly lactide-co-glycolide)纳米缓释微粒,考察其性质及体外释药特性。方法:以PLGA为载体,应用复乳-溶剂挥发法制备P物质缓释纳米粒,并运用酶联免疫吸附法(ELISA)测定其载药率、包封率及体外释药特性。结果:P物质缓释纳米微粒球体均匀度好,平均粒径22.32±5.41 nm,载药纳米球的载药率(0.66±0.036)%;包封率为(66.28±3.56)%。纳米粒体外释药突释期累积释放率为28.65%,12 d后释放率为77.46%。结论:建立了较好的P物质PLGA纳米缓释微粒粉制备工艺,载药纳米微粒体外具有明显缓释特性。Objective：To prepare SP-loaded PLGA nanoparticles and investigate their general properties, drug release characteristics in vitro, Method：PLGA as carrier, SP drug delivery nanoparticles were prepared by double emulsion-solvent evaporation method. Their drug loading efficiecy , encapsulation rate and drug release characteristics were measured with enzyme linked immunosorbent assay（ELISA）method. Result：The SP-loaded PLGA nanoparticles were spherical, uniform, with mean diameter of 22.32 ± 5, 41 nm. The drug loading efficiency and encapsulation rate of the substance P nanoparti- cles was （0.06 ± 0,036）10^-3 % and（66.28 ± 3.56） % respectively. In the drug release test in vitro, the drug release rate was 28, 65% during the burst release phase and rose to 77.46% at the end of 12 th day, Conclusion： The preparation technology of the SP-loaded PLGA nanoparticles was established and the drug loaded nanoparticles have satisfactory phar- rnaceutical properties and sustained release effect in vitro.

关 键 词：P物质 纳米 药物缓释 PLGA 

分 类 号：R783.5[医药卫生—口腔医学]