复发性口腔念珠菌病患者感染源初探  

Primary study on the source of infection in patients with recurrent oral candidiasis

作　　者：沈雪敏[1] 唐国瑶[1] 庄兴[2] 孙康德[2] 周曾同[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔内科,上海市口腔医学研究所,上海200011 [2]上海交通大学医学院附属第九人民医院检验科

出　　处：《临床口腔医学杂志》2005年第11期693-695,共3页Journal of Clinical Stomatology

基　　金：上海市卫生局基金资助课题(044005)

摘　　要：目的:分析复发性口腔念珠菌病患者感染菌株的同源性,初步探讨同一病人感染反复发作是否缘于复发或再感染。方法:对4例复发性口腔念珠菌病患者口腔的10株念珠菌菌株(P1:004、022;P2:011、020、027、029;P3:007、016;P4:023、024)进行分离纯化鉴定,采用计算机系统辅助随机扩增多态性DNA(random amplifiedpolymorphic DNA,RAPD)分析,获得10株菌株的RAPD指纹图,比较菌株的同源性。结果:10株菌株中9株被鉴定为白念珠菌,1株(023)为热带念珠菌。分离自P1和P3的白念珠菌的RAPD基因型相同;P2的020、027、029号菌株基因型相同,而011号菌株不相同;P4的2株菌株不同种。结论:复发性口腔念珠菌病可能存在两方面病因:继往感染复发和/或再感染新菌株。Objective： To analyze the genetic homology of Candida isolates from patients with recurrent oral candidiasis, Method：Computer-assisted random amplified polymorphic DNA （RAPD） analysis was applied to fingerprint 10 oral Candida strains（Pl ： 004,022; P2 ： 011,020,027,029; P3 ： 007,016; P4 ： 023,024） isolated from 4 patients suffering from recurrent oral candidiasis. The genetic homology of the strains were analyzed by the DNA fingerprinting map. Result： Nine strains were identified as Candida albicans and the other one was Candida tropicalis. There was high similarity between RAPD fingerprints of strains from P1 and P3 respectively. And there was also high similarity between strains 020, 027,029 from P2, however, strain 011 was different. The two strains from P4 were different species. Conclusion： There may be two reasons for the happening of recurrent oral candidiasis： recurrence and/or reinfection.

关 键 词：复发性口腔念珠菌病 白念珠菌 基因分型 口腔黏膜病 

分 类 号：R781.5[医药卫生—口腔医学]