白藜芦醇诱导肿瘤细胞凋亡和细胞周期阻滞的作用及机制  

Resveratrol induced apoptosis and cell arrest in cancer cells

作　　者：朱青[1] 张王刚[1] 王立锋[2] 王芳[2] 杨安钢[2] 

机构地区：[1]西安交通大学医学院第二医院血液内科 [2]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710033

出　　处：《第四军医大学学报》2005年第21期1935-1937,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30200369);陕西省科学技术发展计划项目(2003K10-G39)

摘　　要：目的:探讨白藜芦醇(RES)诱导人肝癌HepG2、人慢粒K562肿瘤细胞凋亡和细胞周期阻滞的作用及机制.方法:应用形态学方法,Annexin V荧光染色检测HepG2,K562细胞凋亡的发生,应用流式细胞术(FCM)检测细胞周期,应用四唑蓝比色法(MTT)检测HepG2,K562肿瘤细胞对,RES的药物敏感性.结果:RES对人肝癌HepG2细胞的生长有明显的抑制作用,并诱导肿瘤细胞发生凋亡.凋亡细胞表现为细胞固缩,核染色质碎裂.Annexin V荧光染色检测HepG2细胞凋亡率为33.7%,用FCM检测细胞周期明显阻止于G1期,而K562细胞凋亡率为9.97%.MTT检测表明RES对人肝癌HepG2细胞有剂量-效应关系.但RES对人K562细胞的生长无明显的抑制作用.结论:RES通过诱导HepG2细胞凋亡抑制肿瘤的生长,并有剂量-效应关系.同时RES对肿瘤细胞的抑制及诱导凋亡的作用有细胞选择性.AIM： To study the mechanism of resveratrol induced HepG2 and 1（562 cells apoptosis and cell arrest. METHODS： Cell morphological method and Annexin V technology were used to detect HepG2 and 1〈562 cells apoptosis, flow cytometry （FCM） was used to detect the cell cycle and MTT was used to detect the medicine sensitivity of HepG2 and K562 cells. RESULTS： Apoptosis was seen in human liver cancer HepG2 cell treated with resveratrol and nuclear chromatine condensation and fragmentation were observed in HepG2 cells. Typical ladder patterns of DNA fragmentation were also observed. The highest rate of HepG2 cell apoptosis was 33.7% by Annexin V. Cell cycle stopped at S phase and cell apoptosis rate was 9.97% by FCM. Time and dose dependent effects of resveratrol to HepG2 cell were detected by MTT. Resveratrol had no effect on K562 cells. CONCLUSION： Resveratrol induces HepG2 cell apoptosis and inhibits the development of human liver cancer with a time and dose dependent effect. The resveratrol-induced cell apoptosis is specific to cancer cells.

关 键 词：白藜芦醇 细胞凋亡 细胞周期 HEPG2 K562 

分 类 号：R730.2[医药卫生—肿瘤]