伪狂犬病毒诱导感染乳仔猪扁桃体淋巴细胞发生凋亡  

Induction of apoptosis of the lymphocytes in tonsil of suckling piglets infected with pseudorabies virus

作　　者：石德时[1] 兰盛银[1] 李祥敏[2] 金梅林[2] 徐珍秀[1] 陈焕春[2] 

机构地区：[1]华中农业大学生命科学技术学院分子细胞生物学研究室,湖北武汉430070 [2]华中农业大学动物医学院动物病毒室,湖北武汉430070

出　　处：《电子显微学报》2005年第5期533-536,共4页Journal of Chinese Electron Microscopy Society

基　　金：国家"863计划"基金资助项目(No.2001AA213051);湖北省自然科学基金资助项目(No.2D01ABD110)

摘　　要：将伪狂犬病毒Ea株,按100 TCID50的剂量感染15日龄的健康乳仔猪,所有实验组仔猪在接种后5～7天出现伪狂病症状,取感染组及对照组仔猪扁桃体,进行透射电镜观察、原位末端脱氧核苷酸标记等试验.透射电镜观察发现扁桃体的淋巴细胞发生凋亡,DNA原位末端脱氧核苷酸标记(TUNEL)呈阳性.15 day-old piglets was inoculated with 100 TCID50 of PrV Ea strain each piglet. Transmission electron microscopy（TEM） and in situ terminal-deoxynucleotidyl transferase-mediated dUTP nick end-labeling（TUNEL） staining were applied to analyze the pathological changes. Apoptosis of lymphocytes in tonsil were found under TEM and a large number of TUNEL positive cells were seen in the tonsil tissue.

关 键 词：PRV Ea株 电镜 细胞凋亡 乳仔猪 TUNEL 

分 类 号：S858.28[农业科学—临床兽医学]