高脂饮食性脂肪肝模型大鼠动态病理学观察  

Observation on Dynamic Pathology of Fatty Liver Model Rat Induced by High Fat Diet

作　　者：潘华[1] 姚菊芳[1] 朱萍妹[1] 敖红[1] 周光兴[1] 

机构地区：[1]复旦大学实验动物科学部,上海200032

出　　处：《实验动物与比较医学》2005年第2期83-85,128,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的研究大鼠高脂饮食性脂肪肝模型建立过程中的动态病理学变化规律。方法取体重180-220g清洁级雄性Wistar大鼠60只,根据体重随机分为六组,每组10只,1、2、3组为正常对照组,给予普通饲料;4、5、6组为模型组,给予高脂饲料(93.8%基础饲料,1%胆固醇,5%猪油,0.2%胆盐),分别于第4、8、12周处死1与4组、2与5组、3与6组,并采样作肝脏病理学分析。结果模型组肝指数4组显著高于5、6组和对照组,5、6组均显著低于对照组;组织学观察显示肝细胞脂滴形式早、中、晚三期分别为以细颗粒型、小泡形、大泡形为主且空泡面积依次显著增高;超微结构观察结果显示,模型动物肝脏早期粗面内质网数量多而滑面内质网数量少,中期粗面内质网数量略有减少但滑面内质网呈短管状且数量略有增加,后期粗面内质网数量多而滑面内质网数量少,线粒体略有肿胀。结论在高脂饮食性脂肪肝模型评价中,应以脂滴类型、空泡面积、粗面内质网与滑面内质网数量及形状为评价指标,而肝指数与线粒体变化缺乏作为脂肪肝病理形态评价指标的依据。Objective To explore the sequential pathological changes during the process of establishing high fat diet fatty liver model in rats. Methods Sixty male Wistar rats （180-220 g） were divided randomly into six groups： normal control Groups （1,2,3） fed with common feed and model groups（4,5,6） fed with high fat feed （93.8% foundation feed, 1% cholesterol,5% lard,0.2% bilesalt ）for 4,8,12 weeks. Results The liver index of the model group 4 is significantly higher than those of group 5,6 and normal control groups;but the liver index of the model group 5 , 6 is significantly lower than which of the control groups. The histological observation shows that the form of lipid droplet of the liver cell is mainly grain type, small bubble, large bubble in early, medium and advanced stage with the area of the vacuole significantly increasing one by one .The ultrastructural observation on the liver of the model groups＇ rats shows that the quantity of RER and SER is significantly different in each stage. Conclusion The lipid droplet form , area of vacuole ,quantity of RER and SER should be the evaluating indicator in the estimation of high fat diet fatty liver model, but the liver index and the variety of chondriosome lacks to be.

关 键 词：脂肪肝 模型 动态病理学 大鼠 评价指标 

分 类 号：R575.5[医药卫生—消化系统]