大鼠局灶脑缺血再灌注模型的研究  

A study on the model of focal cerebral ischemia-reperfusion in wistar rats

作　　者：杨世方[1] 闵宝珍[1] 周锡英[1] 

机构地区：[1]河北省医学科学院病理生理研究室

出　　处：《中风与神经疾病杂志》1996年第1期15-17,共3页Journal of Apoplexy and Nervous Diseases

基　　金：河北省自然科学基金

摘　　要：对尼龙线栓塞大鼠大脑中动脉的局部脑缺血再灌注模型进行研究。观察了大鼠脑缺血不同时间再灌注的成功率、脑水肿、神经病学评分及血流量（ｒＣＢＦ）的动态变化。结果如下：（１）改进实验方法后，使大鼠脑缺血不同时间再灌注的成功率明显提高；（２）脑缺血后早期再灌注，可显著降低脑水肿，改善神经损伤症状；（３）大鼠脑缺血期ｒＣＢＦ降至正常值的１５．７％～１９．１％。再灌后，ｒＣＢＦ恢复至正常值的５９．４％～８４．９％。With the rat suture model, we studied the successful rate of reperfusion(SRR) in the rats subjected to ischemia and measured the brain edema of the rats, which underwent 1 (group 1), 3(group2), 6 (group3) or 24 hours (group4) of cerebral ischemia, and were killed at 24 hour after the onset of ischemia.The neurologic deficit scoure were examinated at 3, 6 and 24 hour. We also determined the regional cerebral blood flow (rCBF)in the rats subjected to 3 hours of cerebral ischemia followed by 3 hours of reperfusion. The results showed that:(1)The SRR at 1, 3 and 6 hour after ischemia were 75% , 57% ,and 46%. By improving the model, we increased it to 87% , 77% and 70 %. (2)Both the brain edema and the neurologic deficit score of group 1 or group 2 decreased significantly compared with group4 (P<0.05,P<0.001 respectively). There were no significant difference between group3 and group4 (P>0.05). (3)During 3 hours of sic8hemia, the rCBF was reduced to 15. 7%to 19. 1% of the normal value. During 3 hours of reperfusion, it returned to 59. 4% to 84. 9% of the normal value.

关 键 词：动物模型 脑缺血 脑水肿 再灌注 脑血流量 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R364.12[医药卫生—临床医学]