内毒素作用下肺动脉内皮细胞ET－1基因表达与释放  

ENDOTOXIN STIMULATES THE EXPRESSION AND RELEASE OF ENDOTHELIN-1 IN CULTURED ENDOTHELIAL CELLS

作　　者：汪盛贤[1,2,3] 于忠和 陈志远[1,2,3] 马布仁[1,2,3] 陈玉琼 陈意生[1,2,3] 雷士译 

机构地区：[1]成都军区成都总医院 [2]北京军区总医院 [3]第三军医大学

出　　处：《解放军医学杂志》1996年第1期30-32,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：应用斑点杂交、放免电测定等方法检测分析了内毒素不同浓度同时相和内毒素同浓度不同时相作用下体外培养的牛肺动脉内皮细胞ＥＴ－１基因表达与释放。结果表明，内毒素具有促进内皮细胞ＥＴ－１基因表达和释放的作用，且该作用有明显的剂量依赖关系和时间效应关系；两组ＥＴ－１基因表达的强弱与释放量的增减均呈显著的正相关。提示内毒素血症时血浆ＥＴ水平升高与ＥＴ合成增加和基础释放量增高有密切关系。In this experiment, we investigated the gene expression of endothelin-1 (ET-1) and the release of ET-1 by dot blotting and radioimmunoassay in cuItured bovine pulmonary arterial endothelial cells(PAEC)stimu lated by E coli endotoxin of various concentration(group A) and at various time intervals(group B) in vitro. The resuIts indicated that endotoxin was effective in promoting ET-1 gene exprssion and ET-1 release on a dose and time dependent way from PAEC ET-1 gene expression had a positive correlation with release of ET-1 in group A and group B. These findings suggested that increase of plasma ET-1 Ievels in endotoxemia may be associated with increase of ET-1 synthesis and release.

关 键 词：肺动脉 基因表达 内皮细胞 内皮素 内毒素 

分 类 号：R363[医药卫生—病理学]