奥曲肽联合特异性COX-2抑制剂对肝癌细胞的抑制作用  

Combination of octreotide with specific cyclooxygenase-2 inhibitor enhances the inhibitory effect on growth of human hepatocellular carcinoma

作　　者：俞谦[1] 孙为豪[2] 刘顺英[1] 欧希龙[1] 曹大中[1] 苏菡[1] 江洁[1] 俞婷[1] 

机构地区：[1]东南大学附属中大医院消化科,江苏南京210009 [2]南京医科大学第一附属医院老年医学科,江苏南京210029

出　　处：《东南大学学报（医学版）》2005年第6期363-367,共5页Journal of Southeast University(Medical Science Edition)

摘　　要：目的:研究特异性环氧化酶-2(COX-2)抑制剂NS-398和生长抑素类似物奥曲肽联合使用对人肝癌细胞系SMMC-7721增殖、凋亡的影响。方法:采用四氮唑盐比色法(MTT法)观察细胞增殖活力改变,流式细胞仪检测细胞凋亡百分率。结果:(1)MTT法显示NS-398和奥曲肽均在一定范围内呈浓度和时间依赖性地抑制SMMC-7721细胞的增殖;联合用药组抑制率显著高于单用NS-398或奥曲肽组(P<0.01),金正均方法显示q>1.15,提示两药联合应用有协同抑制肝癌细胞增殖效应,并随着作用时间延长而增强。(2)流式细胞仪测定显示,NS-398、奥曲肽和两药联合作用于SMMC-7721细胞24 h后,联合用药组细胞凋亡率显著高于单一用药组(P<0.01)。结论:NS-398和奥曲肽呈剂量、时间依赖性地抑制SMMC-7721细胞增殖,促进SMMC-7721细胞凋亡,两者联合应用具有协同作用。Objective To investigate the effects of a special COX-2 inhabitor （NS-398） and somastotatin analog （octreotide） on the proliferation and apoptosis in a HCC cell line, SMMC-7721 ; tofocus on observing the effects of combination treatment of NS-398 and octreotide on antiproliferation and induction of apoptosis in SMMC-7721. Methods Cell growth and proliferation of SMMC-7721 were analyzed with MTr assay; apoptosis was detected with flow cytometry assay. Results （1） NS-398 and octreotide inhibited cell growth of SMMC-7721 in a time- and dose-dependent manner. The combination of 1 × 10^-5 mol·L^-1 NS-398 and 1 × 10^-6mol· L^-1 ectreotide inhibited the proliferation more remarkably than either agent applied singularly （P 〈 0.01）, analyzation with Jin Zheng mean square method suggested that q 〉 1.15, and the inhibition rate enhanced along with the time. （2）In apoptosis cell group, Annexin V positive cells could be observed with flowcytometry after treatment of NS-398 of 1 × 10%^-5 mol· L^-1, octreotide of 1 ×10^-6 mol· L^-1 and the combination of the two group for 24 h respectively. The apoptosis rate of combination group was much greater than that when either one was applied singularly （P 〈 0.01）. Condusion NS-398 and octreotide inhibit SMMC-7721 from growing in a time- and dose-dependent manner; both NS-398 and octreotide induce the apoptosis of SMMC-7721 remarkably; the combination of the two has a synergistic effect.

关 键 词：NS-398 奥曲肽 肝癌 细胞增殖 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R73-361[医药卫生—临床医学]