胆总管狭窄对大鼠肝组织BR-UDPGT基因表达的影响(英文)  

EFFECT OF CBD STRICTURE ON THE EXPRESSION OF THE BILIRUBIN UDP-GLUCURONOSYLTRANSFERASE IN RAT LIVER TISSUES

作　　者：张瑞明[1] 高权荣[1] 沈韬[2] 周孝思[2] 

机构地区：[1]内蒙古医学院附属医院,内蒙古呼和浩特010050 [2]北京大学第三医院普外科

出　　处：《内蒙古医学院学报》2005年第3期165-168,172,共5页Acta Academiae Medicinae Neimongol

基　　金：国家自然科学基金资助项目(39670710)

摘　　要：为研究胆总管狭窄时肝组织BR-UDPGT基因表达的改变。本研究用雄性Wistar大鼠不完全结扎胆总管造成胆总管狭窄动物模型。采用Northern印迹杂交和测定BR-UDPGT活性的方法,研究胆总管狭窄对肝组织BR-UDPGT基因表达的影响。结果显示:在胆总管狭窄后12 h^14 d的不同时间段,肝组织BR-UDPGT在mRNA水平均未见基因表达较正常对照组降低。但BR-UDPGT酶活性在胆总管狭窄后12 h后即开始下降,且随狭窄时间的延长BR-UDPGT活性逐渐下降。胆总管狭窄确可引起肝组织BR-UDPGT的活性下降。作用机制不在BR-UDPGT的基因转录水平,可能在转录后的其他环节上。BR-UDPGT活性下降可能是胆总管狭窄后胆汁中的UCB浓度增高的原因之一。This essay is aimed to study the change of the expression of BR-UDPGT. The elevated concentration of unconjugated bilirubin （UCB） in bile plays a crucial role in the pathogenesis of PS. It is well known that the bile duct stricture is one of the factors related to pathogenesis of PS. In the PS animal model induced by common bile duct （CBD） stricture, neither biliary infection nor enhanced activity of β-glucuronidase （β-G） were identified, whereas the concentration of UCB in bile was markedly elevated and PS formed. Therefore, in order to further study the pathogenesis of PS, it is necessary to investigate the change of BR- UDPGT after CBD stricture. Adult male Wistar rats were divided into control and several CBD stricture groups. The expression of BR-UDPGT was investigated by Northern blot and measuring the activity of BR- UDPGT. During the period of 12 hours to 14 days after CBD stricture, the expression of BR-UDPGT in mRNA was almost the same as that of the control group. But the activity of BR-UDPGT was reduced 12 hours after CBD stricture, from 14. 82 μmol/g （protein） /h ± 0. 45 μmol/g （protein） /h to 11.67 μmol/g （protein） / h ± 1.26 μmol/g （protein） /h, （x^-±s, both n = 5, P〈0. 01）. And it became much lower with the duration of CBD stricture. CBD stricture decreased the activity of BR-UDPGT and reduced the ability of liver tissues to transfer UCB into CB. The level affected by CBD stricture was not the transcription of BR-UDPGT. Other steps after gene transcription may be involved. The decreased activity of BR-UDPGT may be one of the causes of elevated UCB concentration after CBD stricture.

关 键 词：肝组织 酶活性 胆红素 基因表达 

分 类 号：R575.7[医药卫生—消化系统]