磁性葡聚糖顺铂微球靶向栓塞治疗大鼠肝癌的研究  

Embolismic study of targeted embolization in rats primary hepatic carcinoma modlels by cis-dichlorodiammine platinum dextran microspheres

作　　者：徐青[1] 陈玉泉[2] 陈易人[1] 徐剑锋[1] 马利林[1] 

机构地区：[1]南通大学附属医院普外科,南通226001 [2]苏州大学附属医院普外科

出　　处：《南通大学学报（医学版）》2005年第5期324-326,共3页Journal of Nantong University(Medical sciences)

摘　　要：目的:研究CDDP-MDMs对Wistar大鼠肝癌模型的靶向栓塞治疗效应.方法: 设立肿瘤区外加磁场组(A组)、无磁场组(B组)、单纯CDDP治疗组(C组)及空白对照组(D组),A、B组以粒径20～50μm自制CDDP-MDMs进行栓塞治疗,研究栓塞前后CT 和肝动脉造影的影像改变及病理变化,检测肿瘤及非靶区肝组织中CDDP-MDMs含量,治疗后肝静脉血中CDDP含量.结果: A组治疗30分钟肝动脉造影肿瘤染色消失,10天后未见再通;CT下肿瘤周围强化明显.肿瘤组织中CDDP-MDMs含量明显高于非靶区肝组织.B组肿瘤组织及正常肝组织中CDDP-MDMs变化不明显.CDDP-MDMs治疗并外加磁场组肝静脉血在治疗后36小时仍维持高CDDP浓度,明显优于C组.结论: CDDP-MDMs 在外磁场的引导下具有良好的靶向性及栓塞作用,可有效提高CDDP-MDMs在肿瘤局部的集聚,微球中药物在大鼠体内具有缓释作用,CDDP-MDMs对肝癌模型具有理想的治疗效果.Objective： A study was made on the effect of CDDP - MDMs （ Cisplatin Magnetic Dextran microspheres） for the targetable embolismic treatment of hepatic carcinoma in Wistar rats. Methods ：Such groups were set up as the tumor zone with additional magnetic field （Group A）, the non- magnetized field （Group B）, the mere CDDP treatment （Group C） and the blank control group （Group D）. For Group A ＆ B, the locally prepared 20 -50um diameter CDDP - MDMs were applied for the embolism treatment so as to study CT and radiographic image variations and pathological changes before and after the arterial embolism, and measure the content of CDDP-MDM in the hepatic tissue of both the tumor and the non-target area as well as that of CDDP in the hepatic venous blood after the treatment. Results：For Group A, the hepatic artery radiography showed that the tumor chromatin would disappear 30 minutes after the treatment and no passage was seen of the artery 10 days after; CT shows obvious intensification on the periphery of the tumor. It is conspicuous that the content of CDDP - MDMs in the tumor tissue of Group A is higher than that of the untargeted area; There was no distinct change of CDDP - MDMs in both the tumor tissues and normal hepatic tissues for Group B. 36 hours after the treatment of CDDP - MDMs combined with additional magnetic field, it was discovered that the CDDP concentration level in the hepatic vein blood remains high, which was better than Group C. Conclusion：CDDP- MDMs, with positive targetabe and embolismic function under the guidance of the external magnetic field, can effectively gather themSelves around the tumor locally; the microsphere - borne drugs have the slow and sustained release effect and CDDP - MDMs are ideally effective in treating hepatoma models.

关 键 词：原发性肝细胞癌 磁性微球 葡聚糖 顺铂 栓塞治疗 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]