地骨皮中生物活性成分的毛细管区带电泳-安培检测方法研究  

Study on Bioactive Ingredients inCortex Lyciiby Capillary Zone Electrophoresis with Amperometric Detection

作　　者：楚清脆[1] 傅亮[1] 林淼[1] 叶建农[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《分析化学》2005年第11期1611-1614,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20375013);华东师范大学2004年优秀博士生培养基金资助项目

摘　　要：采用毛细管区带电泳-安培检测法(CZE-AD)同时分离测定了中药地骨皮中刺槐素、莨菪亭、山奈酚、对香豆酸、香草酸、木犀草素和槲皮素等多种生物活性成分, 考察了运行液酸度和浓度、分离电压、氧化电位和进样时间等实验参数对分离、检测体系的影响.在最佳实验条件下,以直径300 μm的碳圆盘电极为工作电极,检测电位为950 mV(vs. SCE),在80 mmol/L的硼砂缓冲溶液(pH 9.0)中,7组分在24 min可实现基线分离.7组分峰电流与其浓度在1.0×10-3～1.0×10-1范围内呈良好线性,检出限(S/N=3)达3.5×10-5～6.0×10-5 g/L.该方法成功地应用于中药地骨皮中生物活性成分的含量检测.A high-performance capillary zone electrophoresis with amperometric detection （CZE-AD） method has been developed for the determination of bioactive ingredients in Cortex lycii, including acacetin, scopoletin, kaempferol, p-coumaric acid, vanillic acid, luteolin and quercetin. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on CZE-AD were investigated. Under the optimum conditions, the seven analytes could be well separated within 24 min in a 75 cm length capillary at the separation voltage of 16 kV in a 80 mmol L-1 borax running buffer （ pH 9.0）. Good linear relationship was established between peak current and concentration of analytes over three orders of magnitude with detection limits （S/N=3） ranged from 3.5 × 10^-5 to 6.0 × 10^-5 g/L for all analytes. This proposed method has been successfully applied for the study on the electrochemical bioactive ingredients in polar extract of Cortex Lycii, and the assay results were satisfactory.

关 键 词：毛细管电泳 电化学检测 地骨皮 电化学特性图谱 毛细管区带电泳 生物活性成分 安培检测法 最佳实验条件 分离测定 氧化电位 

分 类 号：O658.9[理学—分析化学] O657.1[理学—化学]