低浓度的丝裂霉素对结肠癌光动力增效作用研究  

Synergistic effect of low-concentration of Mitomycin C on photodynamic therapy of colon carcinoma cell

作　　者：廖新华[1] 陈祖林[2] 葛海燕[2] 梁平[2] 

机构地区：[1]第三军医大学基础医学部物理学教研室,重庆400038 [2]第三军医大学新桥医院普通外科,重庆400037

出　　处：《第三军医大学学报》2005年第20期2054-2056,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30271481)~~

摘　　要：目的用低浓度的丝裂霉素改变大肠癌细胞周期来达到增强其光动力学疗法,探讨增效作用中对癌细胞杀伤作用规律。方法在结肠癌细胞培养的基础上,加入低浓度的丝裂霉素,光动力学疗法后采用荧光光度法和MTT法对癌细胞内光敏剂含量和细胞存活率进行检测,用SPLM软件统计包进行数据处理。结果低浓度的丝裂霉素可使癌细胞内光敏剂显著增加,随时间延长细胞内光敏含量增加明星;细胞内光敏剂含量与激光照射后细胞存活率呈显著负相关,即细胞内光敏剂含量多,PDT后细胞存活率低;光动力学疗法后癌细胞存活率曲线明显左移,在4、8、16h细胞存活率显著降低;癌细胞与光敏剂作用8～16h激光照射可达到最佳的光动力杀伤效果。结论我们设计单独低浓度的丝裂霉素对结肠癌细胞无杀伤作用,但可通过增加癌细胞内光敏剂含量来达到增效作用,8～16h为最佳给药时间,杀伤作用有明显的规律性。Objective To study the synergistic effects of Mitomycin C in photodynamic therapy for colonic carcinoma. Methods The colon carcinoma cell line SWS00 was assigned to be treated with 0. 02 μg/ml Mitomycin C, or 2 μg/ml PSD-007 （photosensitizer） , or Mitomycin C and PSD-007 （PSD ＋ MC group）. The cells without any special treatment served as control. Fluorescence spectrophotometer and MTF assay were used tO detect the content of intracellular photosensitizer and survival rate of SW700 cells under photodynamic therapy. The obtained data was analyzed with SPLM statistical software. Results After PSD was added for 4, 8, 16 and 24 h, intracellular PSD level of PSD ＋ MC group increased significantly as compared with that of PSD group. The intracellular PSD contents of PSD group, PSD ＋ MC group had a strong and negative correlation with cell survival rate respectively after photodynamic therapy, but the cell survival rate curve shifted to the left obviously. The best killing effects on SW700 cells of photodynamic therapy could be obtained when the SW700 cells had been exposed to photosensitizer for 8 - 16 h. Conclusion There is insignificant influence of lowconcentration of Mitomycin C alone on cell survival rate. The better synergistic effect of Mitomycin C on killing the colon carcimoma cells can be achieved by enhancing the intracellular content of photosensitizer for 6-8 h before photodynamic therapy.

关 键 词：光动力学疗法 结肠肿瘤 丝裂霉素C 

分 类 号：R730.5[医药卫生—肿瘤] R735.35[医药卫生—临床医学]