肺鳞癌INK4a/ARF基因启动子甲基化研究  

The study of INK4a/ARF gene promoter methylation in bronchial squamous cell carcinomas(SCC).

作　　者：高丽莉[1] 胡义德[1] 刘丽红[1] 

机构地区：[1]第三军医大学附属新桥医院全军肿瘤中心,重庆400037

出　　处：《四川医学》2005年第11期1190-1192,共3页Sichuan Medical Journal

基　　金：国家自然科学基金项目(30170293);重庆市科委应用基础项目[2001]资助

摘　　要：目的分析肺鳞癌(SCC)及其周围正常肺组织INK4a、ARF基因启动子甲基化改变情况。方法收集外科手术切除标本SCC标本17例,同时选取周围正常肺组织作为对照,运用甲基化特异性PCR(MSP)方法对癌组织及其周围正常肺组织INK4a和ARF基因启动子的甲基化状态进行检测。结果17例SCC组织,13例INK4a扩增阳性,2例ARF扩增阳性;对应的正常肺组织,1例INK4a扩增阳性,1例ARF扩增阳性。卡方检验,INK4a在肺癌组织和正常肺组织之间差异有显著性,ARF在肺癌组织和正常肺组织之间差异无显著性。结论INF4a基因启动子甲基化是SCC的一个普遍性事件,参与SCC的形成过程,可作为SCC的分子诊断标志之一,而ARF基因甲基化在SCC则是一个相对罕见性事件。Objective To study INK4a/ARF promoter methylation in bronchial squamous cell carciomas（SCC） .Methods 17 SCC and its surrounding normal lung tissues were chosed to study the INK4a/ARF methylation with the method of MSP（ methylation-specific PCR）. Results Among 17 SCC, INK4a methylaion were found in 13 patients, in contrast, INK4a methylation were found in only 1 of their surrounding normal lung tissues（ P 〈 0.01 ） .Meanwhile,2 patients were found ARF methylation in 17 SCC but 1 patient in surrounding normal lung tissues （ P 〉 0.05 ）. Conclusion INK4a gene methylation is a common phenomenon while ARF methylafion is rarely occurred in SCC.

关 键 词：肺鳞癌 INK4a/ARF基因 甲基化 甲基化特异性PCR 

分 类 号：R734.2[医药卫生—肿瘤]