高危型HPVDNA检测在宫颈不典型鳞状细胞再分类中的意义  

Role for High-risk HPV DNA Testing in Reclassification of ASC

作　　者：郭会芹 [1] 潘秦镜 [1] 李香菊 [1] 曹箭 [1] 张洵 [1] 章文华 [2] 吴令英 [2] 陈凤 [3] 乔友林 [3] 

机构地区：[1]100021,北京,中国协和医科大学中国医学科学院肿瘤医院病理科 [2]100021,北京,中国协和医科大学中国医学科学院肿瘤医院妇瘤科 [3]100021,北京,中国协和医科大学中国医学科学院肿瘤医院流行病室

出　　处：《肿瘤防治研究》2005年第11期733-735,共3页Cancer Research on Prevention and Treatment

摘　　要：目的明确高危型人乳头瘤病毒基因(highriskhumanpapillomavirusDNA,HRHPVDNA)检测在宫颈不典型鳞状细胞(atypicalsquamouscell,ASC)再分类中的意义,为其临床应用提供依据。方法我院2002年于山西省阳城县和襄垣县进行的“中国山西宫颈癌普查方法研究”项目中,9034名妇女均进行了液基细胞学检查、HRHPVDNA检测和5%醋酸染色肉眼观察。我们对其中细胞学诊断为ASC,伴有HRHPVDNA检测阳性或5%醋酸染色肉眼观察异常的病例进行了阴道镜检查及活检,比较HRHPVDNA阳性ASC组和HRHPVDNA阴性5%醋酸染色肉眼观察阳性ASC组中宫颈上皮内瘤变(Cervicalintraepithelialneoplasia,CIN)的发生率,从而评价HRHPVDNA检测作为ASC再分类方法的可行性。结果在进行阴道镜检查的548例ASC中,HRHPVDNA阳性者有333例(60.8%),HRHPVDNA阴性肉眼观察阳性者215例(39.2%)。这两组ASC中CIN的发生率分别为22.5%和5.1%(P<0.05);CIN2/3的发生率分别为7.8%和0.9%(P<0.05)。结论HRHPVDNA检测是ASC再分类的有效方法。Objective To define the significance of high-risk human papillomavirus DNA （HR-HPV DNA）testing in reclassification of atypical squamous cell （ASC）, and provide scientific basis for its clinical utility. Methods A total of 9034 women were enrolled in a cervical cancer screening program in Shared China, in 2002. In this program, all subjects were examined by liquid-based cytology, HR-HPV DNA testing and unaided visual inspection with acetic acid （5%）. Of them, the cases of ASC with positive HPV DNA and negative HPV DNA-positive unaided visual inspection were taken colposcopic biopsy. We compared the incidence of cervical intraepithelial neoplasia（ClN） between the positive HPV DNA group of AS（2 and the negative HPV DNA-positive unaided visual inspection group of AS（2. Results There were 548 cases of ASC, who were taken colposcopic biopsy. Of the 548 cases,333（60. 8%） cases were HPV DNA-positive, 215（39. 2%） cases were negative HPV DNA-positive unaided visual inspection. In HPV DNA positive group, the incidence of CIN is 22. 5% ,while the incidence of CIN2/3 is 7. 8%. Both were significantly higher than that of negative HPV DNA-positive unaided visual inspection group （5. 1 % and 0. 9%）. Conclusion HR-HPV DNA testing is a feasible method for reclassification of AS（2.

关 键 词：宫颈上皮内瘤变 不典型鳞状细胞 杂交捕获Ⅱ代技术 

分 类 号：R737.330.2[医药卫生—肿瘤]