细小病毒H-1感染对胃癌细胞核修复相关基因表达的影响  

Impacts of Parvovirus H-1 Infection on the Expressions of DNA Repair Related Genes in Gastric Cancer Cells

作　　者：冯缨[1] 冉志华[1] 刘炯[1] 萧树东[1] 

机构地区：[1]上海第二医科大学仁济医院上海市消化疾病研究所,上海200001

出　　处：《上海第二医科大学学报》2005年第10期1018-1021,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家教委青年骨干教师基金;德国大众基金(grantⅠ/75890)资助项目

摘　　要：目的探讨细小病毒H-1诱导胃癌细胞死亡的可能机制。方法选取对细小病毒H-1敏感和不敏感的胃癌细胞株HGC27和BGC823,H-1病毒感染48 h后提取细胞总RNA。应用不同标记的dCTP逆转录制备荧光探针,与含有8 000点人类体细胞基因序列的表达谱cDNA芯片杂交,计算机荧光扫描分析HGC27细胞核修复相关基因表达谱的改变。实时定量PCR检测HGC27和BGC823细胞部分核修复相关基因的表达。结果基因芯片分析显示,HGC27细胞的64对核修复相关基因中,12对基因表达水平下调至0.5以下,6对基因表达水平上调至2倍以上。PCR定量检测证实,HGC27细胞BTG2表达明显下调,MBD2表达显著上调,XRCC4和H2A.Z的表达无明显改变;BGC823细胞BTG2表达明显下调,XRCC4、H2A.Z和MBD2表达无明显改变。结论细小病毒H-1可能通过影响胃癌细胞核修复通路相关基因的表达,使细胞基因转录或细胞周期停止,从而诱导胃癌细胞死亡。Objective To probe into the possible mechanism of gastric cancer cell death induced byparvovirus H-1. Methods The H-1 virus-sensitive gastric cancer cell line HGC27 and insensible cell line BGC823 were employed in this study. Cellular RNA was extracted 48 h after H-1 virus inoculation. The differentially labelled fluorescent dCTP revdrse transcription generated probes were used to hybridize with cDNA genechip containing 8 000 spots of human somatic gene sequences. The fluorescence of genechip was scanned by a specialized computer, and the changes of DNA repair related gene expression profiles were further analyzed in HGC27 cells. The expressions of some DNA repair related genes were further verified by real-time PCR in HGC27 and BGC823 cells. Results The cDNA microarray results indicated that 12 out of 64 DNA repair related genes in HGC27 cells were down-regulated （ inferior to 0. 5 fold） and 6 genes was up-regulated （superior to 2 folds）. The results of real-time PCR amplification displayed that the expression of BTG2 was markedly decreased while MBD2 was increased, and the expression of XRCC4 and H2A. Z had no apparent change in HGC27 cells. However, in BGC823 cells, the expression of BTG2 was distinctly increased, whilst the expression of XRCC4, H2A. Z and MBD2 had no apparent change. Conclusion The study suggests that parvovirus H-1 may influence the expression of DNA repair pathways related genes to regulate gene transcription and cell cycle progression to induce gastric cancer cell death.

关 键 词：胃癌 细小病毒H-1 核修复 基因表达 

分 类 号：R735.2[医药卫生—肿瘤] Q756[医药卫生—临床医学]