复方三根制剂配合瘤内注射DDP调控荷肺癌小鼠Bcl-2、P53基因表达的实验研究  

Research on the Expressions of Gene Bcl-2 and P53 in Mice Bearing Lewis Lung Cancer After Administered with DDP by Intratumor Injection and FFSG Mixture by Oral

作　　者：黄玉军[1] 舒琦瑾 冯正权 王泽时[3] 方剑文[3] 

机构地区：[1]浙江省嵊州市中医院,嵊州312400 [2]浙江中医学院附属医院 [3]浙江中医学院

出　　处：《浙江中医学院学报》2005年第6期50-51,54,共3页Journal of Zhejiang College of Traditional Chinese Medicine

摘　　要：目的观察与探讨Bcl-2及P53基因在荷肺癌小鼠经复方三根制剂配合瘤内注射治疗后的表达情况。方法用lewis肺癌瘤株造模于38只C57BL/6近交系小鼠右腋下,8天后将其随机分成2组A组(13只)、B组(12只)、C组(13只),并分别予以A组复方三根制剂加瘤内注射DDP治疗,B组单纯DDP瘤内注射治疗,C组瘤内注射NS对照。21天后处死所有小鼠并取下肿瘤标本作HE染色及免疫组化染色检测,观察Bcl-2、P53表达情况。结果Bcl-2表达情况A组为46.1%,B组为58.3%,C组为61.5%;P53表达情况A组为38.4%,B组为41.67%,C组69.2%。结论瘤内注射DDP可以通过降低Bcl-2、MTP53表达途径促进肿瘤细胞凋亡,而中药复方三根制剂能协同DDP进一步下调两者的表达。Objective：To observe the statement of gene Bcl-2 and P53 in mice bearing lewis lung cancer after administered DDP by intratumor injection and FFSG mixture by oral. Methods： To prepare model mice of c57b1/6 planted lewis lung cancer. The models were randomly divided into three groups, marked group A, group B and C in 8 days. Two groups were treated with DDP by intratumor injection and FFSG mixture by oral and simply administered DDP by intratumor injection respectively. Group C was administered DDP by intratumor injection as control. After administered 21 days, all mice were killed and the samples of tumor were removed. The state of gene Bcl-2 and P53 in samples was measured by immunology history biology method. Results：The positive rates of states of Bcl-2 are 46. 1%（A）, 58.3%（B） and 61.5% （C） respectively . There are significant differences between A and C. The positive rates of states of P53 are 38.4%（A）, 41.67%A（B） and 69.2%（C） respectively. There are also significant differences between A or B and C. Conclusion：DDP by intratumor injection may promote tumor apoptosis through regulating down Bcl-2 and P53 pathway,and these functions can be facilitated by a FFSG mixture.

关 键 词：复方三根制剂 瘤内注射 肺癌 BCL-2 P53 基因表达 

分 类 号：R735.2[医药卫生—肿瘤] R734.2[医药卫生—临床医学]