Expression and Mutation of MAGE-A3 mRNA in Lung Cancer Tissues  

肺癌组织中黑色素瘤抗原-A3基因mRNA的表达及其序列点突变分析(英文)

作　　者：巴月[1] 李志远[1] 何薇薇[1] 吴皓[1] 程学敏[1] 庄东刚[1] 吴逸明[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生与职业病教研室,450052

出　　处：《The Chinese-German Journal of Clinical Oncology》2005年第5期287-291,325,共6页中德临床肿瘤学杂志（英文版）

基　　金：This project was supported by the key project of Scientific Committee of Henan Province (No. 0124170232), the key project ofZhengzhou Scientific Committee (No. 04BA60ABYD18), and Tumor Biology Subproject of 211 project of zhengzhou Univevsity

摘　　要：Objective： To inwvetigate the expression of MAGE-A3 mRNA in tissue samples derived from lung cancers and to discuss the possibility of using MAGE-A3 antigens as a new peptide vaccine for inunotherapy for lung cancers. Methods： Tumor tissue samples of lung cancers and paired non-tumor tissues of the lung were obtaimed from 31 lung cancer patients. Total RNA was extracted and cDNA was synthesized. Nested polymernse chain reaction amplification using MAGE-A3 specific primer was performed to detect the expression of MAGE-A3. The 10 clones of 5 samples of MAGE-A3 mRNA positive PCR products were DNA sequenced by using DNAs sequencer （PE-377）. Results： Of 31 lung cancers, 26 （83.9%） expressed MACE-A3 mRNA. The expression of MAGE-A3 gene was not detectable in the adjacent lung tissues. The DNA sequencing confirmed that the target gene fragment in all 5 samples of PCR products was MACE-A3 cDNA. Point nmtations occurred in 4 samples （8 clones） detected （C^2773→T^2773; G^2807→A^2807） resulting in alternation of amino acid residue in one position （E^143→K）. Conclusion： （1） The MAGE-A3 gene was expressed exclusively in tumor tissues of the patients with lung cancer in China. This tumor rejection antigen may have potential to be used as a new peptide vaccine for immunotherapy for lung eancers. （2） There are two point mutations of MAGE-A3 gene sequence in some Chinese lung cancer patients.

关 键 词：MAGE-A3 mRNA：lung cancer tumor antigen 

分 类 号：R734.2[医药卫生—肿瘤] R739.5[医药卫生—临床医学]