舒林酸、尼美舒利对人胃癌SGC-7901细胞增殖及COX-2、NF-κB表达的抑制作用  

Inhibitory Effects of Sulindac and Nimesulide on Cell Proliferation and COX-2,NF-κB Expression in Human Gastric Carcinoma Cell Line SGC-7901

作　　者：朱祖安[1] 刘莹[2] 费素娟[1] 

机构地区：[1]徐州医学院附属医院消化内科,221000 [2]徐州医学院病理学教研室

出　　处：《肿瘤防治研究》2005年第10期623-626,672,共5页Cancer Research on Prevention and Treatment

基　　金：江苏省教育厅自然科学研究计划项目(01KJB320011)

摘　　要：目的探讨舒林酸、尼美舒利对人胃癌SGC-7901细胞增殖及COX-2、NF-κB表达的影响。方法对人胃癌SGC-7901细胞采用细胞培养,MTT法检测不同浓度舒林酸、尼美舒利对人胃癌SGC-7901细胞增殖抑制作用,免疫组化、western blot方法检测药物对COX-2、NF-κB表达的影响。结果舒林酸、尼美舒利可以抑制人胃癌SGC-7901细胞生长并具有浓度、时间依赖性;与阴性对照组相比,药物作用48h后尼美舒利100、200μmol/L组,舒林酸0.6、1.2mmol/L组COX-2、NF-κB蛋白表达明显减弱并具有剂量依赖性(P<0.05),COX-2、NF-κB之间正相关(P<0.05)。结论舒林酸、尼美舒利可以抑制人胃癌SGC-7901细胞增殖,抑制NF-κB,COX-2的蛋白表达在二者抑制肿瘤机制中可能具有一定作用。Objective To investigate the inhibitory effects of sulindac and nimesulide on cell proliferation and COX-2, NF-κB expression in human gastric carcinoma cells. Methods SGC-7901 ceils were treated by sulindac and nimesulide. The anti-proliferative effect was measured by using MTT assay;COX-2 and NF-κB protein expression were examined by immunohistochemical stain and western-blot analysis. Results Tumor cells proliferation was inhibited in dose and time dependent manner. The expression of COX-2, NF-κB in 100, 200μmol/L nimesulide groups and 0. 6,1.2mmol/L Sulindac groups were significantly decreased than the negative group（P〈0.05）. There was positive correlation between COX-2 and NF-κB protein expression （P〈0. 05）. Conclusion The proliferation of SCX2-7901 cells was inhibited after treated by sulindac and nimesulide. Suppressing COX-2, NF-κB expression may play some roles in the antitumor process of these two drugs.

关 键 词：舒林酸 尼美舒利 人胃癌SGC-7901细胞 核因子κB 环氧合酶2 

分 类 号：R73-361[医药卫生—肿瘤] R735.2[医药卫生—临床医学]