低频脉冲磁场对大鼠学习与记忆、EEG及脑组织形态学的影响  

Effects of Low-frequency pulsed electromagnetic field on learning , memory, EEG and brain tissue morphology in rats

作　　者：李伯勤[1] 张鸿日[2] 张向红[1] 王旭平[3] 彭静华[2] 张保华[1] 邢子英[1] 彭勃[4] 

机构地区：[1]山东大学医学院解剖与组织胚胎学研究所,山东济南250012 [2]山东大学齐鲁医院神经外科,山东济南250012 [3]山东大学医学院细胞生物学研究所,山东济南250012 [4]山东大学控制科学与工程学院,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第10期924-927,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2001C28)。

摘　　要：目的:研究低频弱脉冲磁场(LFPMF)对大鼠学习和记忆、脑电图(EEG)及脑组织形态学的影响。方法:选用雄性SpragueDawley(SD)大鼠36只,随机分为对照组(A组)和磁场辐射组(B组),每组各18只;B组施加低频弱脉冲磁场(频率15Hz,平均场强0.1mT)30d,1次/d,45min/次;两组均采用Morris水迷宫方法测试逃避潜伏期;用光镜和电镜进行形态学观察;RM600八导脑电图仪检测脑电图。结果:第1次辐射24h后,B组大鼠行为改变明显,逃避潜伏期明显延长,差异有统计学意义(P<0.05);随着时间延长,两组之间的差别逐渐减少,无统计学意义(P>0.05);脑电图显示,B组大鼠的频率和波幅均低于A组(P<0.05);其脑组织形态学存在异常改变。结论:采用低频弱脉冲磁场长时程辐射,应充分考虑其负面影响。Objective： To investigate the effect of LFPMF on learning , memory, EEG and brain tissue morphology in rats. Methods ：Thirty-six adult male Sprague Dawley（SD） rats were grouped into control and irradation randomly （n=18）. Rats in irradation group were exposed to 0.1 mT intensity LFPMF of 15 Hz for 45 minutes per day for 30 days. The influence of LFPMF on the neurons were detected by using Morris maze test, and the morphology and the electroencephalogram （EEG） were observed by fluorescence microscope,microscopy and EEG instrument. Results： Rats in irradation group took more time to acquire the taskin the maze, especial in the first day （P〈0.05）; the amplitude and the frequency of the EEG were significantly lower in irradation group than control （P〈0.05）, which showed negative effect of the exposure to the LFPMF; abnormal changes of neurons were observed under the light and the electronic microscope in irradation group. Conclusion：The negative effect should be taken into account when irradiating with LFPMF is adopted.

关 键 词：低频弱脉冲磁场 学习 记忆 脑电描记术 大鼠 Sprague Dawley 

分 类 号：Q689[生物学—生物物理学]