应用鼠白血病病毒L6565建立小鼠艾滋病模型  

A neonatal mouse model of AIDS induced by L6565 murine leukemia virus

作　　者：贾敏[1] 杨铁虹[1] 姚秀娟[1] 孟嘉[1] 梅其炳[1] 

机构地区：[1]第四军医大学药理学教研室,陕西西安710032

出　　处：《中国药理学通报》2005年第10期1271-1273,共3页Chinese Pharmacological Bulletin

基　　金：陕西省中医管理局资助项目(No0347)

摘　　要：目的应用小鼠L6565白血病病毒建立小鼠艾滋病模型,并在此模型上观察抗艾滋病药物双汰芝的治疗作用。方法用小鼠L6565白血病病毒感染出生24 h内的乳鼠。以RT-PCR法测定血浆病毒载量;流式细胞术测定CD4+、CD8+细胞的百分数;镜检计数外周血白细胞、红细胞和骨髓有核细胞数;测定脾脏、胸腺的重量并计算脏体系数,石蜡切片作组织病理检查。结果与正常对照组相比,模型组小鼠血浆病毒载量较高,CD4+细胞百分率、CD4+/CD8+比值、红细胞和骨髓有核细胞数以及胸腺系数均降低,而白细胞数和脾脏系数升高;双汰芝组血浆病毒载量与模型组相比降低,CD4+细胞的百分数提高,同时降低脾脏指数、升高胸腺指数;改善脾脏、淋巴结的病理改变,却降低模型动物白细胞数,并进一步降低RBC和骨髓有核细胞数。结论用L6565白血病病毒株复制的小鼠艾滋病模型出现了类似人艾滋病的临床症状,提示其可以作为评价抗艾滋病药物疗效的一种小动物模型。Aim To establish a mouse model of AIDS and observe the effects of Combivir, an anti-AIDS drug. Methods The model was set up by infecting infant mice with I6565 mufine leukemia virus. Quantitative RT-PCR method was used to measure viral load in plasma. The percentage of CD4^＋ and CD8^＋ lymphacytes was estimated by flowcytometric method. The number of WBC, RBC and marrow karyote was counted, indexes of immune organs were weighed and calculated. Results Compared with control,the viral load in plasma in the model was at high level. The percentage of CD4^＋ lymphocyte, the ratio of CD4^＋/CD8^＋ , the number of RBC and marrow karyote,and thymus index were significantly decreased in the model, while the number of WBC and spleen index was remarkably increased. Conclusion AIDS patient symptom is shown in mouse infected with L6565 murine leukemia virus. This suggests that this model is likely to be used as a mice model to evaluate the effect of antiAIDS drugs.

关 键 词：艾滋病 逆转录病毒 免疫调节 

分 类 号：R-332[医药卫生] R363-332