匹罗卡品诱导癫痫大鼠内源性神经干细胞的可塑性改变  

Change of the plasticity of inherent neural stem cells in the epilepsy model induced by pilocarpine in rats

作　　者：笱玉兰[1] 朱遂强[1] 徐金枝[1] 许峰[1] 张广慧[1] 李卫华[1] 阮旭中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《卒中与神经疾病》2005年第5期259-261,293,共4页Stroke and Nervous Diseases

基　　金：湖北省卫生厅科研基金项目(NO.JXIA03)

摘　　要：目的探讨匹罗卡品诱导癫痫模型的内源性神经干细胞的增殖、迁移,从而研究癫痫发生后的神经发生及可塑性改变。方法氯化锂和匹罗卡品联合诱导大鼠癫痫模型,分为对照组、癫痫发作后3、7、14、28 d组,用免疫组织化学方法动态检测大鼠海马多唾液酸神经细胞粘附分子(polysiolylated neural cell ad-hesion molecule,PSA-NCAM)及PSA-NCAM/5-溴脱氧尿苷嘧啶(bromodeoxyuridine,BrdU)的表达。结果与对照组比较,海马齿状回(dentate gyrus,DG)PSA-NCAM及PSA-NCAM/BrdU的阳性细胞在癫痫后3 d开始增加(P<0.05),14 d达到高峰(P<0.05),28 d后开始下降,但仍高于对照组(P<0.05)。结论癫痫发生后出现神经干细胞的增殖、迁移,也即出现了神经发生和可塑性改变。Objective To investigate the proliferation and migration of inherent neural stem cells in the epilepsy model induced by pilocarpine in rats. Methods The generalized seizure （GS） was induced by lithiumpilocarpine. The expression of polysiolylated neural cell adhesion molecule （PSA-NCAM） and PSA-NCAM/ bromodeoxyuridine （BrdU） was observed in hippocampus by immunohisochemistry and immunofluorescence staining. Results Compared with the controls, the number of PSA-NCAM-POsitive cells and PSA-NCAM/BrdU positive cells in hippocampus increased at day 3 after GS（P〈0. 05）, reached maximum at day 14 （P〈 0. 01）,and decreased markedly at day 28, but it was still elevated compared with that of the controls （P〈 0. 05）. All positive cells in bilateral dentate gyrus （DG） were located in the deepest portion of the granule cell layter （GCL）. Conclusions These observations indicate that GS may stimulate the proliferation and migration of inherent neural stem cells,as well as neurogenesis and plastic change of originally-existed neural stem cells may occur in response to the GS.

关 键 词：癫痫 神经干细胞 多唾液酸神经细胞粘附分子 可塑性 

分 类 号：R742.1[医药卫生—神经病学与精神病学]