MSH-PE40重组毒素的构建和抗头颈部肿瘤的实验性研究  

Experimental Study on Construction of Recombinant toxin MSH-PE40 and its anti-tumor activity

作　　者：徐洪君[1] 魏洪涛[1] 朱冬冬[1] 杨占泉[1] 董震[1] 

机构地区：[1]吉林大学中日联谊医院耳鼻喉科,吉林长春130033

出　　处：《中国实验诊断学》2005年第5期680-682,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的构建以绿脓杜菌外毒素A(PE40)为毒性部分的重组毒素,并研究其抗头颈部肿瘤的效应。方法利用基因工程技术将α促黑激素(-αmelanoma stimulating hormone,-αMSH)与绿脓杆菌外毒素A的部分重组基因,克隆到高效表达载体pET-20b中,表达出重组蛋白MSH-PE40,进行头颈部肿瘤细胞的体外杀伤实验。结果成功地将-αMSH基因与PE40基因融合,处于T7启动子和终止子调控之下。初步纯化的MSH-PE40对黑色素瘤具有明显的细胞毒性。结论MSH-PE40具有特异性杀伤-αMSH受体的肿瘤的功能。Objective To construct anti-tumor recombinant toxin with PE4O as toxic part and study its cytotoxic effect. Method Recombinant gene of α-MSH and PF40 using gene recombinant technique. Coloned the α-MSH-PE40 gene into the expression vec tor pET-20b, induced with IPIG and expressed the recombinant protein. Used the recombinant protein to exam the eytotoxin effect i vitro. Results Analysis of digesting with restriction endonueleases and DNA sequencing verified that the junction between two gene had fight reading frame. Conclusion MSH-PE40 have strong toxin to melanoma cell B16, laryngeal carcinoma cell Hep-2, the control groups have no obvious changes.

关 键 词：肿瘤 促黑激素 绿脓杆菌外毒素A 重组毒素 

分 类 号：R739.91[医药卫生—肿瘤]