血管内皮生长因子受体在鼠横纹肌的表达与羟基磷灰石/碳纳米管复合材料毒性研究  

The correlation study of VEGF receptor expression in striated muscles of rat and the toxicity of CNTs/HAP composite

作　　者：赵冬梅[1] 刘中浩[1] 赵敬杰[1] 武士清[1] 李爱民[2] 王彭[3] 孙康宁[2] 赵燕[1] 孙楠[1] 

机构地区：[1]山东大学第二医院骨科,250033 [2]山东大学(南校区)材料学院材料液态结构及其遗传性教育部重点实验室,250061 [3]北京协和医院中心实验室,北京100005

出　　处：《中国生物医学工程学报》2005年第5期587-589,595,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学资助项目基金(30170269)。

摘　　要：目的从观察鼠骨骼肌组织形态学改变和VEGF表达的角度研究CNTs/HAP纳微米复合材料对鼠骨骼肌的细胞毒性。方法将CNTs/HAP纳米复合材料植入实验鼠臀大肌。按不同时间取材,做组织学观察和RT-PCR分析。结果1d、3d显微镜下观察,炎细胞和淋巴细胞浸润伴轻度水肿、间质疏松;5d、7d显微镜下观察血管壁增厚,单核巨噬细胞和纤维组织增生,少量肌纤维出现变性,VEGF表达呈上升趋势;14天显微镜下观察周围组织间质血管扩张,仅有少量炎细胞浸润,水肿不明显,VEGF表达呈下降趋势。结论CNTs/HAP对鼠骨骼肌细胞无毒性,有利于血管的再生,具有良好的生物相容性。Objective To study the cell toxicity of CNTs/HAP implant in white rat＇s gluteus maximus by observation of morphology change and VEGF expression. Method The histology observation and RT-PCR analysis in rat＇s gluteus was studied at different time after the CNTs/HAP composites were implanted. Results After 1 and 3 days the inflammation cells and lymphocyte infiltrated with edematous tissue and loose stroma were observed. After 5 and 7 days the wall of blood vessel grew thick and the mononuclear macrophage and the fibrous tissue proliferated. Small amount of degenerated muscle fiber appeared and the VEGF expression increased. After 14 days the blood vessel of the pripheral fibrous tissue dilated, with a few pologistic cells infiltrating, at the same time tissue edema was not obvious and the VEGF expression decreased. Conclusion The CNTs/HAP composite showed enhancement of blood vessel regeneration and has acceptable biocompatibility.

关 键 词：碳纳米管 骨骼肌 生物相容性 血管内皮生长因子 

分 类 号：R318[医药卫生—生物医学工程]