Ⅲ型胶原mRNA在不同牙合力状态下大鼠磨牙牙周膜中的表达  

Expression of Type Ⅲ Collagen mRNA in Rat Periodontal Ligamemt in Response to Different Occlusal Force

作　　者：范新平[1] 赵云凤[2] 刘卫平[3] 赵筱芩 

机构地区：[1]同济大学附属同济医院口腔科,上海200065 [2]四川大学华西口腔医院修复科 [3]四川大学华西医院病理科 [4]上海市安图医院口腔科

出　　处：《四川大学学报（医学版）》2005年第6期843-846,共4页Journal of Sichuan University(Medical Sciences)

摘　　要：目的从基因转录水平研究不同牙合力状态下Ⅲ型胶原在大鼠磨牙牙周膜中的表达变化及其意义。方法建立大鼠牙合力丧失牙、合创伤的模型,采用原位杂交的方法分别检测在12 h、3 d、7 d、14 d及30 d时牙周膜细胞中Ⅲ型胶原mRNA表达的动态变化。结果与正常牙合力相比,牙合力丧失后牙周膜中Ⅲ型胶原mRNA表达迅速减弱,随后逐渐增强,7 d时最强(P<0.05),其中最大信号平均强度值154.39±17.6,14 d后恢复到正常牙合力时的表达水平;牙合创伤后牙周膜中表达Ⅲ型胶原mRNA的阳性细胞数量和密度逐渐增加,7 d时达到最大,其表达的平均强度为124.03±14.82(P<0.05),14 d后显著减弱,其信号平均强度值为63.07±5.69。结论Ⅲ型胶原mRNA的表达与牙合力的变化密切相关。Objective To investigate the altered expression level of type Ⅲ collagen mRNA in the periodontal ligament of rat molar in response to changes in occlusal force. Mehods A model of the rat experimental occlusal trauma was established, in which the occlusal surface on the upper first molar of （250±20）g male SD rats was approximately 1 mm elevated unilaterally. Another model was establishde, in which the upper first molar was extracted to remove occlusal force against the lower first molar. The rats were perfusion-fixed at 12 h and on the 3rd, 7th, 14th and 30th day after treatment. The prepared sagittal sections were processed to study the expression of type Ⅲ collagen mRNA in rat periondontal ligament by in situ hynbridization. Results Following the bite-raising, the number and the desity of the positive cells of type Ⅲ collagen mRNA in the periodontal ligament of the lower first molar increased gradually. The expression of type Ⅲ collagen mRNA was the strongest on the 7th day （124.03±14.82）. Comparde to the normal rats,the expression level of type Ⅲ collagen mRNA in the periodontal ligament of the lower first molar significantly decreased on the 14th day （63.07±5.69）. When occlusal force was removed the expression level of type Ⅲ collagen mRNA in the periodontal ligament of the lower first molar showed a rapid decrease. Subsequently, a gradual increase was recognized. Compared to the normal animal,a more significant increase（154.39±17.61） was detected on the 7th day after occlusal force was removed. Conclusion The results suggest that type Ⅲ collagen may play important roles in the process of periodontium remodeling against different occlusal force.

关 键 词：Ⅲ型胶原 原位杂交 [牙合]力 

分 类 号：R783[医药卫生—口腔医学]