八棱丝瓜蛋白2对B16细胞的诱导分化作用  

Differentiation-Inducing Effect of Luffaculin-2 on B16 Melanoma Cells

作　　者：谢捷明[1] 许盈[1] 陈明晃[2] 许建华[1] 俞昌喜[1] 赵蓉[3] 吴国华[1] 林建忠[1] 

机构地区：[1]福建医科大学药理学系,福州350004 [2]中国科学院福建物质结构研究所结构化学国家重点实验室,福州350002 [3]福建医科大学分子医学教研室,福州350004

出　　处：《福建医科大学学报》2005年第4期349-352,共4页Journal of Fujian Medical University

基　　金：国家自然科学基金资助项目(39970872);福建省自然科学基金资助项目(C0510012;C0410018);福建医科大学科研基金资助项目(FJGXY04019;2002m012)

摘　　要：目的测定八棱丝瓜蛋白2(LF-2)对小鼠黑色素瘤B16细胞的诱导分化作用。方法以MTT法测定LF-2对B16细胞的增殖抑制作用,以形态学、黑色素含量及细胞周期变化为指标,观察LF-2诱导B16细胞分化的作用。结果LF-2对B16细胞具有明显的增殖抑制作用,并使B16细胞停滞于G1期;细胞生长缓慢,平铺不重叠,甚至形成网状结构,呈正常上皮样细胞分化,细胞质内细胞器丰富,黑色素小体明显增多;黑色素含量显著增加。结论LF-2对B16细胞具有明显的诱导分化作用。Objective To investigate the inducing differentiation effect of luffaculin-2 on B16 mouse melanoma ceils. Methods MTT assay was used to evaluate the inhibitory effect of luffaculin-2 on proliferation of B16 cells. Morphological changes, melanin content and distribution of cell cycle were also used to estimate the differentiation-inducing activity of luffaculin-2 in B16 ceils. Results Luffaculin-2 significantly inhibited the proliferation of B16 cells. Luffaculin-2 interrupted the transition of cell cycle from G1 phase to S phase. With luffaculin-2 treatment, B16 ceils were differentiated into mature epithelia-like ceils, grew slowly in monolayer and even formed reticulation, enriched cell organelles in cytoplasm, increased the number of melanosome obviously, and enhanced the melanin content markedly. Conclusion Luffaculin-2 can significantly induce differentiation of B16 cells.

关 键 词：八棱丝瓜蛋白 黑色素瘤 细胞分化 

分 类 号：R73-3[医药卫生—肿瘤]