聚氧乙烯蓖麻油增强锌酞菁光敏剂对小鼠肿瘤的光动力治疗作用  

Cremophor EL Enhance Znic Phthalocyanine-Mediated Photodynamic Therapy in Murine Cancer

作　　者：翁绳美[1] 孙建成[1] 黄自强[1] 

机构地区：[1]福建医科大学药理学系,福州350004

出　　处：《福建医科大学学报》2005年第4期353-355,共3页Journal of Fujian Medical University

基　　金：国家自然科学基金资助项目(200201005)

摘　　要：目的探讨聚氧乙烯蓖麻油(CrEL)作为磺酸基邻苯二甲酰亚胺甲基锌酞菁(ZnPcS2P2)的溶剂在移植性小鼠肿瘤及体外培养的肿瘤细胞的光动力治疗中的作用。方法荷U14肿瘤的小鼠,尾静脉注射ZnPcS2P224 h后,激光(670 nm)照射,继续饲养5 d后剥取肿瘤称质量;用体外培养的黑色素瘤B16细胞与含或不含CrEL的药物作用2 h后洗去药物,光辐射后继续培养24 h,测定药物对肿瘤细胞的杀伤作用。结果不含CrEL的锌酞菁对小鼠移植瘤的光动力抗癌作用弱,其光动力抗癌作用随CrEL含量增加而增强。Zn-PcS2P2对体外培养的B16细胞的光动力杀伤作用,也明显依赖于CrEL的含量。结论CrEL能增强Zn-PcS2P2的光动力抗肿瘤作用。Objective To survey the effect of cremophor EL（CrEL） for enhance the efficacy of zinc phthalocyanine（ZnPcSp2） photodynamic therapy（PDT） in murine cancer. Methods Znic phthalocyanine in a CrEL formulation was given by tail intravenously infused to bearing a subcutaneously transplanted mouse cervical cancer U14, photodynamic therapy（laser 670 nm, 200 mW/cm^2, 72 J/cm^2） at 24 h after injection. Another experiment in culture melanoma B16 cells, after treatment with the photosensitizer, then washed the drug and CrEL, after light irridiation（ 600 - 750 nm, 4.5 mW/cm^2, 5.4 J/cm^2）, the ceils were cultured for a further 24 h. Cytotoxicity was detected by the method of sulforhodamin B. Results In the U14-bearing mice ex- perimental model, CrEL improved the antitumor activity of ZnPcS2P2（2 mg/kg） according on CrEL concentra- tion. Furthermore, CrEL enhanced in vitro cytotoxicity of Zn-PcS2P2 in cultured B16 cells. Conclusion These findings suggest that CrEL increased ZnPcS2P2 antitumor activity.

关 键 词：锌酞菁 光化学疗法 聚氧乙烯蓖麻油 肿瘤 实验性 小鼠 

分 类 号：R73-36[医药卫生—肿瘤]