高表达热休克蛋白70鼠脑胶质瘤细胞瘤苗的体内抗瘤作用  

Preventive Efficacies of Vaccination with Heat Shock Protein70-Enriched Glioma Cells in Rats

作　　者：林章雅[1] 康德智[1] 连葆强[1] 林元相[1] 苏东辉[2] 

机构地区：[1]福建医科大学附属第一医院神经外科,福州350005 [2]福建医科大学免疫学教研室,福州350004

出　　处：《福建医科大学学报》2005年第4期373-375,共3页Journal of Fujian Medical University

基　　金：福建省教育厅科研基金资助项目(K2001074)

摘　　要：目的观察高表达热休克蛋白70(HSP70)鼠脑胶质瘤细胞(C6细胞)瘤苗在体内的抗瘤效应。方法以SD大鼠为动物模型,采用热休克、β榄香烯、丝裂霉素单独或联合诱导制备不同高表达HSP70表达率的细胞瘤苗。各实验组注射不同HSP70蛋白表达率的瘤苗进行免疫,对照组注射1640培养液;C6细胞皮下注射,观察皮下胶质瘤成瘤率及成瘤大鼠的生存期,分析HSP70蛋白表达率与抗瘤效应间的关系。结果经C6细胞瘤苗免疫大鼠较对照组皮下胶质瘤成瘤率下降,成瘤大鼠平均生存期明显延长,平均生存期与瘤苗的HSP70表达率呈正相关,应用单克隆抗体封闭瘤苗的HSP70表达则此作用消失。结论应用高表达HSP70的C6细胞瘤苗明显延长载瘤大鼠生存期。瘤苗免疫大鼠的抗瘤作用是由HSP70介导的。Objective To evaluate the efficacy of inactivated glioma cells with enriched heat shock protein 70（HSP70） as vaccine to prevent the hosts from developing the homogenous tumor in an experimental animal model. Methods A variety of C6 cell vaccines prepared by heat-shock procedure and/or the treatment of mitomycin C or beta-elemene, or their combinations, were assayed by flow cytometry for their HSPT0 expression and injected into SD rats prior to C6 cell challenge. The injection of RMPI 1640 as control. The rate of glioma formation in combination with the length of survival was analyzed for the preventive effect of vaccines and the length of survival alone for therapeutic efficacy evaluation. The relation between the anti-tumor efficacy and the expression of HSP70 was also analyzed. Results The survival rat of immunization rats was significant increase than that of the control group. The formation of subcutaneouos glioma in immunization rats was significiant decrease than that of control group（P〈0.01）. The efficacies of protection in preventive use of glioma vaccines were found to be closely associated with the levels of HSPT0 antibodies（P 〈 0.01）. Conclusion Vac- cination of HSP70-enriched glioma cells is capable of improving the survival of rats suffered with glioma. The antitumor effect is mediate by HSP70.

关 键 词：热休克蛋白质类 70 神经胶质瘤 热休克反应 细胞 培养的 

分 类 号：R739.4[医药卫生—肿瘤]