角蛋白单克隆抗体AE1/AE3免疫组化法检测胃癌淋巴结微转移的研究  

Detection of lymph node micrometastasis of gastric cancer using monoclonal anti-cytokeratin(AE1/AE3) antibody immunohistochemistry

作　　者：程春生[1] 董卫国[1] 于皆平[1] 罗和生[1] 

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《中国医师杂志》2005年第11期1446-1448,共3页Journal of Chinese Physician

基　　金：湖北省教育厅重点科研计划项目资助(2003x124)

摘　　要：目的探讨AE1/AE3免疫组化标记检测胃癌根治标本中淋巴结内微小转移癌及其临床意义。方法对64例胃癌根治切除后阴性的淋巴结及胃周软组织的石蜡包埋组织用S-P法进行AE1/AE3染色标记。结果64例118枚淋巴结中,根据HE染色阴性再经AE1/AE3标记发现,8例患者13枚淋巴结发现微转移病灶,病例总阳性率12.5%(8/64),淋巴结总阳性率11.02%(13/118)。微转移与浸润深度有关,深层组织的微转移阳性率高于浅层(P<0.05),弥漫型胃癌微转移率(62.50%)明显高于肠型胃癌(5.36%),两者之间具有显著差异性。结论胃癌周围阴性淋巴结中存在微转移灶,AE1/AE3染色标记检测微转移淋巴结可能对准确地确定临床分期、指导治疗、判断预后有积极临床意义。Objective To explore the clinical significance of cytokeratin（ AE1/AE3） immunohistochemical staining in detecting the lymph node micrometastasis of gastric cancer. Methods Immunohistochemical S-P method was tsed to examine AE1/AE3 in 118 lymph nodes in 64 cases of primary gastric cancer. Results Among 118 lymph nodes, micrometastasis was found in 13 lymph nodes of 8 patients with primary gastric cancer by AE1/AE3 staining. The positive rate of micrometastasis was 12. 5% and the positive rate of lymph nodes was 11.02%. The incidence of micrometastasis was related with the depth of invasion, and in the cancer with deep invasion, the positive rate of micrometastasis was higher than that in the cancer with superficial invasion（ P〈0. 05 ）. The incidence of micrometastasis in diffusion invasire gastric cancer（62. 50% ） was much higher than that in intestinal type gastric cancer （5.36%） （ P〈0. 05 ）. Conclusion There is micrometastasis in the lymph nodes around the gastric cancer, and the detection of micrometastasis by AE1/AE3 immunohistochemical staining in the negative lymph nodes of gastric cancer may be helpful for the tumor stage, treatment and prognosis evaluation.

关 键 词：AE1/AE3 胃癌 淋巴结 微转移 

分 类 号：R735.2[医药卫生—肿瘤]