3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮对L-02细胞增殖周期和凋亡的影响  

Effect of 3-chloro-4-(dichloromethyl)-5-hydroxy-2 [5H]-Furanone on Proliferation and Apoptosis of L-02Cells

作　　者：刘爱林[1] 余日安[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,湖北武汉430030

出　　处：《癌变．畸变．突变》2005年第6期340-342,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助项目(No.30170794)

摘　　要：背景与目的:研究饮水氯化消毒副产物3_氯_4_二氯甲基_5_羟基_2(5氢)_呋喃酮(3_chloro_4_(dichloromethyl)_5_hydroxy_2[5H]_Furanone,MX)对人胚胎肝细胞株(Humanderivedf etalhepatocytes,L_02)增殖周期和凋亡的影响。材料与方法:L_02细胞经MX(10、30、100、300μmol/L)染毒3h后继续培养0、3、6、9h,用吖啶橙/溴化乙啶染色法和流式细胞术分析凋亡和增殖周期。结果:L_02细胞在周期不同时相的分布与MX染毒剂量和染毒后继续培养的时间有关;染毒后继续培养0h时,30μmol/LMX诱导S期细胞显著增加,10、100、300μmol/LMX诱导G2期细胞显著增加(P<0.01);在染毒后继续培养的第9h,10μmol/LMX诱导G2期细胞显著增加,30、100、300μmol/LMX诱导S期细胞显著增加(P<0.05)。MX各剂量组L_02细胞的凋亡率,随继续培养时间的延长,呈现先升后降的趋势,在6h时达最大值,与溶剂对照组相比,差异有统计学意义(P<0.01);30μmol/LMX剂量组L_02细胞的凋亡率最高。结论:MX可诱导L_02细胞增殖周期G2期、S期阻滞和凋亡,细胞周期阻滞的类型与MX染毒剂量和染毒后继续培养的时间有关。BACKGROUND ＆ AIM： To study the effect of 3-chloro-4-（dichloromethyl）-5-hydroxy-2[5H]-furanone （MX） on proliferation and apoptosis of human-derived fetal hepatocytes（L-02） cells. MATERIAL AND METHODS： L-02 cells were treated with MX （10,30,100,300 μmol/L）for 3 hours and further incubated for 0, 3, 6 and 9 hours. Proliferation and apoptosis of L-02 cells were analyzed by using flow cytometric analysis and microscopic analysis after staining with ethidium bromide and acridine orange. RESULTS： The distribution of cells at different phases of the cell cycle was associated with the dose of MX and the incubation time. When L-02 cells were treated with MX for 3 hours and not further incubated, S phase and G2 phase cells increased significantly at MX concentration of 30 μmol/L and 10, 100, 300 μmol/L, respectively. At incubation time of 9 hours , G2 phase and S phase cells increased significantly at MX concentration of 10 μmol/L and 30,100, 300 μmol/L,respectively. The percentage of apoptotic L-02 cells increased at first and decreased subsequently with further incubation time. Peak value of apoptotic percentage appeared at the incubation time of 6 hours and showed statistical significance in comparison with solvent control （P 〈 0.01）. The highest apoptotic percentage was observed at the MX concentration of 30 μmol/L. CONCLUSION： MX induced apoptosis and arrested cycling of L-02 cells at G2 phase and S phase. The type of cell cycle being arrested was associated with the dose of MX and the incubation time.

关 键 词：3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮 人胚胎肝细胞株 凋亡 细胞周期 

分 类 号：R394.6[医药卫生—医学遗传学] R994.6[医药卫生—基础医学]