光动力作用对膀胱癌细胞线粒体相关调控蛋白Bcl-2/Bax表达的影响  

Effect of photodynamic therapy with laser-activated BPD-MA on the expressions of mitochondria-related proteins Bcl-2 and Bax of human bladder cancer BIU-87 cells

作　　者：许川山[1] 王志刚[1] 吴士明[2] 虞乐华[3] 

机构地区：[1]重庆医科大学超声影像学研究所,重庆400010 [2]第三军医大学新桥医院红外研究中心,重庆400037 [3]重庆医科大学附属第二医院康复医学科,重庆400010

出　　处：《第三军医大学学报》2005年第22期2219-2222,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30300468);全军医学科研"十五"计划青年基金资助(01Q102)~~

摘　　要：目的探讨激光活化BPD-MA光动力诱导肿瘤细胞凋亡的可能机制。方法应用免疫组织化学染色检测激光活化BPD-MA光动力作用后人膀胱癌细胞B IU-87线粒体相关调控蛋白Bc l-2和Bax蛋白表达水平。结果激光活化BPD-MA光动力作用后膀胱癌细胞线粒体相关调控蛋白Bc l-2表达显著低于对照组(P<0.05),而Bax蛋白表达水平无明显改变(P>0.05),但Bax/Bc l-2比值较对照组明显上升(P<0.05)。结论激光活化BPD-MA光动力作用后人膀胱癌细胞B IU-87线粒体相关调控蛋白Bc l-2表达水平的下降、Bax/Bc l-2比值的上升。激光活化BPD-MA光动力作用可能通过调控线粒体相关调控蛋白Bc l-2和Bax蛋白表达水平诱导人膀胱癌细胞株B IU-87发生凋亡。Objective To study on the mechanisms that photodynamic therapy with laser-activated BPD-MA induces human bladder cancer BIU-87 cell apoptosis. Methods Human bladder cancer BIU-87 cells at log phase were randomized into 4 groups. The cells of BDP-MA-PDT groups were added with BPD-MA and then activated with 632.8 nm He-Ne laser; PDT group without BDP-MA addition was only irradiated; BDP-MA group without irradiation; normal control group without BDP-MA addition or irradiation. Photosensitization of BPD-MA was activated by laser with red light （632.8 nm） delivered at 10 mw/cm^2 to give a total dose of 2.4 J/cm^2. The expression of Bcl-2 and Bax proteins in human bladder cancer BIU-87 cells were detected by immunohistochemical staining. Results The Bcl-2 protein expression in BPD-MA-PDT group was lower than that of other groups （P 〈 0.05 ） , and no significant difference in Bax protein expression between the BPD-MA-PDT group and other groups （P 〉0.05 ） , therefore the ratio of Bax to Bcl-2 obviously increased （P 〈0.05 ）. Conclusion Photodynamic therapy with laser-activated BPD-MA can down-regulate the Bcl-2 protein expression and increase the ratio of Bax to Bcl-2, which may be one of the mechanisms of apoptosis triggered by photodynamic therapy with lser-activated BPD-MA.

关 键 词：激光 光动力作用 膀胱癌细胞 细胞凋亡 BCL-2蛋白 BAX蛋白 

分 类 号：R394.2[医药卫生—医学遗传学] R730.23[医药卫生—基础医学]