移植脊髓中嗅鞘细胞的生物学特性(英文)  

Biological characteristics of olfactory ensheathing cells after transplantation into spine cord

作　　者：马元琛[1] 唐勇[2] 沈慧勇[2] 杨志华[3] 吴燕峰[4] 王学文[2] 王鹏[2] 

机构地区：[1]广东省人民医院骨科,广东省广州市510080 [2]中山大学附属第二医院骨科,广东省广州市510120 [3]广州医学院附属第一医院神经科,广东省广州市510182 [4]中山大学附属第二医院岭南脐血库,广东省广州市510120

出　　处：《中国临床康复》2005年第42期131-133,i0003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:嗅鞘细胞被证明有修复损伤脊髓的作用,但对其移植后的生物学特性了解不多。目的:观察移植的嗅鞘细胞在损伤脊髓中的迁移方式。设计:随机对照实验。单位:广东省人民医院骨科,中山大学北校区动物实验中心。材料:2个月雄性SD大鼠38只,体质量(350±20)g。方法:实验于2004-02/05在中山大学北校区动物实验中心进行。①取SD大鼠2只用于嗅鞘细胞提取,将嗅鞘细胞用双苯亚甲胺染色。②取SD大鼠36只,随机分为3组,近端组,远端组和对照组,每组12只。近端、远端组建立大鼠胸髓损伤模型。分别于脊髓损伤近端、远端注入嗅鞘细胞悬液;对照组不损伤胸髓,仅注射嗅鞘细胞悬液。主要观察指标:术后1,2,4,6周荧光显微镜下观察脊髓中嗅鞘细胞的分布。结果:36只大鼠,分为3组,术后近端组死亡1只;远端、对照组各死亡2只。进入结果分析29只。各组大鼠脊髓中嗅鞘细胞分布:术后2,4,6周,近端组和远端组的嗅鞘细胞进一步沿着脊髓长轴纵向迁移,最远达8mm,能够穿过瘢痕,到达断端对侧的细胞数量很少;对照组嗅鞘细胞只是局部的扩散,没有迁移。结论:嗅鞘细胞的迁移主要沿着轴突进行,向头端和尾端迁移的数量和速度都没有明显区别,嗅鞘细胞只在损伤的脊髓中进行迁移。BACKGROUND：Olfactory ensheathing cells （OECs） have been shown to possess the potential of repairing injured spinal cord, but their biological characteristics after transplantation in vivo are not well understood.OBJECTIVE： To investigate the migration of OECs after transplantation into the injured spinal cord of adult rats.DESIGN： Randomized and controlled experiment.SETTING： Department of Orthopedics, Guangdong Provincial People＇s Hospital; Experimental Animal Center of the North Campus of Sun Yat-sen UniversityMATERIALS： Totally 38 2-month-old male SD rats with body mass of （350 ±20） g were used in this study.METHODS： This experiment was conducted in the Experimental Animal Center, North Campus of Sun Yat-sen University between February 2004and May 2004. Two SD rats were used to extract the OECs, which were stained with Hoechst 33342. Totally 36 SD rats were subsequently randomized into 3 groups, namely rostral transplantation group, caudal transplantation group and control group with 12 rats in each group. The rats in the rostral and caudal transplantation groups subjected to operations to establish thoracic spinal cord injury model and OEC suspension was injected; in the control group, the rats were spared of thoracic spinal cord injury with only OEC suspension injection.MAIN OUTCOME MEASURES： Distribution of OECs in the spinal cord was observed under fluorescence microscope 1, 2, 4 and 6 weeks after operation, respectively.RESULTS： Of the rats in the 3 groups, 1 died in the rostral group, and 2in each of the caudal transplantation and control groups, leaving 29 rats for result analysis. The OECs in the rostral and caudal transplantation groups migrated longitudinally along the long axis of the spinal cord to a farthest distance of 8 mm and penetrating the scar tissues, but very few cells could reach the contralateral side. The OECs in the control group diffused locally without migration.CONCLUSION： OECs mainly migrate along the axons in white matter of the injured spinal cord, an

关 键 词：嗅神经 脊髓损伤 移植 

分 类 号：R394.2[医药卫生—医学遗传学]