一氧化氮合酶抑制剂对大鼠缺氧缺血性脑损伤神经元凋亡的影响  

The Effect of Nitric Oxide Synthase Inhibitor on Apoptosis of Neurons after Hypo xic-Ischemic Brain Damage in Rats

作　　者：姚瑜[1] 俞惠民[2] 余钟声[2] 

机构地区：[1]浙江大学医学院附属第二医院,杭州310009 [2]浙江大学医学院附属儿童医院,杭州310003

出　　处：《科技通报》2005年第6期689-692,696,共5页Bulletin of Science and Technology

基　　金：浙江省自然科学基金资助项目(398428)

摘　　要：目的研究神经元凋亡在大鼠缺氧缺血性脑损伤(HIBD)中的地位及作用。并探讨选择性一氧化氮合酶抑制剂—7-硝基-吲唑(7-NI)对大鼠HIBD时神经元凋亡的影响。方法24h时用HE染色、TUNEL法、透射电镜观察和检测神经元凋亡并评估7-NI对神经元凋亡的影响。结果HIBD后24h在海马、皮质等处检测到神经元凋亡。7-NI使海马及皮质部位的神经元凋亡数显著降低。结论神经元凋亡参与了HIBD后神经元迟发性死亡。7NI有显著的抗HIBD后神经元凋亡的作用。Objective To study the status and contribution of neuronal apoptosis after hypoxic-ischemic brain damage （HIBD） in rat. And to evaluate the effect of selective nitric oxide synthase inhibitor--7-NItroindazole （7-NI） on apoptosis of neurons after HIBD in rats. Methods HE staining, TUNEL staining and transmission electron microscopy were used to detected neuronal apoptosis 24 hours after HIBD. The effect of 7-NI on neuronal apoptosis was also studied. Results Neuronal apoptosis was detected in hippocampus and cortex at 24 h after HIBD. 7-NI can significantly reduced neuronal apoptosis after HIBD. Conclusions It is suggested that apoptosis account for important role of the delayed neuronal death after HIBD. It is selective NOS inhibitors--7-NI that shows its anti-apoptosis effect on HIBD 24 hours later.

关 键 词：神经病学 凋亡 脑损伤 缺氧缺血 7-硝基-吲唑 

分 类 号：R741[医药卫生—神经病学与精神病学]