eIF4E、p53在喉癌、切缘及良性病变中表达的相关性研究  

Study of the correlation about the expression of eIF4E and p53 in laryngeal squamous cell carcinoma、margin and benign tissues.

作　　者：罗文政[1] 张剑[2] 江红群[2] 文三立[2] 张琨和[2] 祝金泉 

机构地区：[1]江西省肿瘤医院头颈外科,江西南昌330000 [2]江西医学院第一附院,江西南昌330000

出　　处：《现代肿瘤医学》2005年第6期753-755,共3页Journal of Modern Oncology

摘　　要：目的探讨原癌基因真核细胞翻译起始因子eIF4E在喉鳞癌中的表达及其与临床病理特征的相关性。方法选能手术切除的喉鳞癌病人及喉良性病变做对照每组各20例,应用W estern b lot法检测eIF4E表达同时免疫组化检测p53表达作对照。结果eIF4E在喉鳞癌中心组织普遍存在程度不等的高表达,其中心癌组织和边缘癌组织阳性指数分别为6.41±1.27、4.39±1.12,二者存在显著差异,良性病变正常低表达;不同病理分型Ⅰ、Ⅱ,Ⅲ级的eIF4E阳性指数分别为5.50±1.25、7.68±0.95,二者存在显著性差异,不同部位、不同TNM分期亦存在显著性差异;p53在喉鳞癌中阳性表达率为55%(11/20)p53阳性表达率与病理分型相关,与病变部位无关,良性病变阴性表达。结论eIF4E在喉鳞癌中普遍高表达,对喉鳞癌的发生、发展及临床恶性程度、预后判断有重要意义,p5 3在喉癌中5 5%过表达,其与喉癌的发生发展预后相关,但缺乏普遍性。Objective To study the correlation of elF4E in the clinical pathological characteristics of laryngeal carcinoma and understand the expressive characteristics and clinical significance of eIF4E.p53 in the laryngeal squamous cell carcinoma. Methods Twenty cases laryngeal carcinoma that can be underwent operation and 20 cases benign tissues were selected to carry out experiment . Using Western blot method to detect the expression of eIF4E and immunohistochemistry to detect p53. Results All 20 LSCC tissue overexpressed elF4E in the central and marginal carcinoma6.41 ± 1.27,4.39±1.12. A normally low expression was observed in normal mucosa,lO samples of vocal cord polyps and chronic hypertrophic laryngitis. There was a significant correlation between overexpression of protein eIF4E with pathological grade,clinical stage,the site of tumors（ P 〈 0.01 ） ,in addition, there was a significant difference between overexpression degree of elF4E with central and marginal carcinoma. In LSCC,there were a high expression of p53 protein（55%, 11/20） ,which was related to the clinical stage, pathological grade;but not to the site of tumors. Conclusion There was a general overexpression of protein 4E in larngeal carcinoma. The eIF4E play an important role on tumorgenesis,development and invasion of LSCC. There is an important significance in the anlysis of maligment degree of ISCC. In LSCC,55% p53 protein exists overexpression,which was related to the clinical stage and pathological grade,but not related to the site of tumors.

关 键 词：喉鳞癌 切缘标本 良性病变 EIF4E P53 

分 类 号：R739.65[医药卫生—肿瘤]