壳聚糖-顺铂微球瘤内治疗大鼠C6胶质瘤的实验观察  

Experimental Observation of Intratumoral Chemotherapy of Chitosan-cisplatin Microspheres in C6 Glioma Rats

作　　者：徐海涛[1] 黄书岚[1] 陈谦学[1] 马金阳[1] 

机构地区：[1]武汉大学人民医院神经外科,武汉430060

出　　处：《武汉大学学报（医学版）》2005年第6期743-746,共4页Medical Journal of Wuhan University

摘　　要：目的:观察壳聚糖-顺铂缓释微球对C6胶质瘤瘤内化疗的疗效。方法:将C6细胞株进行传代、培养,并对C6胶质瘤细胞进行顺铂体外药敏测定,然后制作动物脑胶质瘤模型,造模后7 d沿原创道分别给予生理盐水、顺铂及壳聚糖-顺铂缓释剂10μl,观察各组大鼠瘤内给药后的生存期,并取病理标本行病理学检查及免疫组织化学染色检测。结果:C6胶质瘤细胞对顺铂敏感;胶质瘤模型成瘤率为100%;壳聚糖-顺铂缓释剂组平均生存期为(36.47±9.96)d,明显长于生理盐水组(16.80±6.36)d和顺铂组(28.64±10.14)d(P<0.05)。结论:壳聚糖-顺铂微球的瘤内化疗疗效明显,且比单纯顺铂用药效果好。Objective： To demonstrate the efficacy of intratumoral chemotherapy of chitosan-cisplatin microspheres on C6 glioma rats. Methods： C6 glioma cells were passaged and cultured. Pharmacodynamics of cisplatin treating C6 glioma cells in vitro was identified. Glioma-loaded animal models were established by inoculating C6 glioma cells to caudate nucleus of rats. Seven days later, rats respectively received injection of 10 μ1 physiological saline, cisplatin and chitosan-cisplatin microspheres. Then the survival time was observed individually. After the rats were dead, pathological samples were selected immediately for pathological examination and immunohistochemical staining. Results： C6 glioma cells were sensitive to cisplatin and grew in all rats. Mean survival period of chitosan-cisplatin microspheres group was （36.47 ±9.96）d, which was longer than that of physiological saline group and cisplatin group（16. 80±6.36）d, （28.6±10.14）d respectively, P〈0.05）. Conclusion： The efficacy of intratumoral chemotherapy via chitosan- cisplatin microspheres to C6 glioma rats is obvious, and better than single cisplatin administration.

关 键 词：壳聚糖 顺铂 微球 瘤内化疗 C6细胞株 

分 类 号：R739.41[医药卫生—肿瘤]