Cox-2抑制剂对骨肉瘤细胞及乳腺癌细胞生长的抑制作用  

Inhibitory Effects of Cyclooxygenase-2 Specific Inhibitor on Human Osteosarcoma and Breast Cancer Cell Line

作　　者：孙敬华[1] 蔡林[1] 陈方舟[1] 张克勇[1] 屈雪菊[2] 

机构地区：[1]武汉大学中南医院骨外科,武汉430071 [2]武汉大学医学结构生物学研究中心实验室,武汉430071

出　　处：《武汉大学学报（医学版）》2005年第6期766-769,共4页Medical Journal of Wuhan University

摘　　要：目的:观察Cox-2抑制剂(塞来昔布、罗非昔布)对人骨肉瘤细胞株HOS-8603、乳腺癌细胞株MDA的作用。方法:采用MTT比色法测定体外培养的细胞株HOS-8603、MDA-MB-435S对不同浓度的罗非昔布和塞来昔布的敏感性。流式细胞仪分析细胞增殖周期的影响。结果:选择性环氧化酶-2(Cox-2)抑制剂明显抑制HOS-8603、MDA-MB-435S的生长(P<0.05),抑制效应呈现浓度依赖型和时间依赖型,HOS-8603在Cox-2抑制剂作用下,细胞分裂阻滞在G2/M期,有一定的诱导细胞发生凋亡作用,塞来昔布强于罗非昔布对HOS-8603的作用。结论:选择性环氧化酶-2抑制剂对骨肉瘤细胞、乳腺癌细胞的生长可能起着十分重要的作用,有望成为成骨肉瘤治疗和抑制骨肉瘤转移的新药物。Objective： To compare the effects of two kinds of selective cyclooxygenase-2 inhibitors （celecoxib, rofecoxib） on the growth of human osteosarcoma and breast cancer cell line. Methods： The anti-proliferative effect was measured by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry. Results： Cyclooxygenase-2 specific inhibitor had a growth inhibitory and apoptosis-inducting effect on three kinds of tumor cell. The inhibition effects showed a dose-dependence and time-dependence manner. MTT assay indicated a significant difference between the rofecoxib and celecoxib group. The cells treated with Cyclooxygenase-2 specific inhibitors showed G2/M arrest. Moreover, celecoxib was more inhibitorily higher than rofecoxib. Conclusion： Cyclooxygenase-2 specific inhibitors have significant effect on the growth of two kinds of cell line. Cyclooxygenase-2 specific inhibitors may become one of the important medicines in the treatment and metastasis of tumor.

关 键 词：环氧化酶-2 骨肉瘤 乳腺癌 细胞培养 

分 类 号：R738.1[医药卫生—肿瘤]