太白洋参对青年和老年大鼠血清中过氧化脂质、单胺氧化酶、超氧化物歧化酶的影响(英文)  

Effect of taibai yangshen on serum lipid peroxide, monoamine oxidase, and superoxide dismutase of young and old rats

作　　者：雷国莲[1] 王薇[2] 金颖[1] 王继涛[1] 颜永刚[2] 

机构地区：[1]陕西中医学院药学系,陕西省西安市712083 [2]陕西中医学院

出　　处：《中国临床康复》2005年第39期182-183,共2页Chinese Journal of Clinical Rehabilitation

基　　金：陕西教育厅专项科研计划项目(01JK095)~~

摘　　要：背景:过氧化脂质、单胺氧化酶、超氧化物歧化酶是测定体内自由基清除能力的重要生化药理指标。过氧化脂质还可作为机体衰老氧化的一个定量指标。目的:通过测定衰老模型大鼠血清中单胺氧化酶、过氧化脂质及超氧化物歧化酶活性,验证太白洋参的抗自由基作用。设计:随机对照实验。单位:陕西中医学院药学系。材料:实验于2002-03/04在陕西中医学院药理实验基地完成。选择健康SD青年、老年大鼠各40只,太白洋参药材粉碎用水提取,并将滤液浓缩至1.5g/mL。人参用水煎煮提取并浓缩至0.3g/mL。方法:青年大鼠和老年大鼠随机分为8组:青年正常对照组、青年人参水煎液组、青年太白洋参大剂量组和青年太白洋参小剂量组。老年正常对照组、老年人参水煎液组、老年太白洋参大剂量组和老年太白洋参小剂量组,每组10只。适应环境7d后,正常对照组每日用20g/kg蒸馏水灌胃,人参水煎液组用人参水煎液按4g/(kg·d)灌胃,太白洋参大剂量组和小剂量组分别用20g/(kg·d),10g/(kg·d)灌胃,35d后于麻醉状态下断头取血处死。收集血清,离心后采用硫化巴比妥酸法测定丙二醛浓度、采用紫外吸收法测定单胺氧化酶活性、采用黄嘌呤氧化酶法测定超氧化物歧化酶活性。主要观察指标:青年和老年大鼠血清丙二醛浓度、单胺氧化酶、超氧化物歧化酶活性。结果:80只大鼠均进入结果分析。①人参水煎液组和太白洋参大、小剂量组的丙二醛含量和单胺氧化酶活性均低于正常对照组(P<0.05～0.01),超氧化物歧化酶活性高于正常对照组(P<0.05～0.01)。②老年太白洋参小剂量组大鼠血清中丙二醛含量和单胺氧化酶活性高于青年太白洋参小剂量组大鼠[(8.23±1.73),(6.73±1.69)μmol/L;(41.7±15.9),(40.9±14.6)nkat/L,P<0.05],而老年太白洋参大、小剂量组超氧化物歧化酶活性低于青年太白洋参大、小剂量大鼠[(3BACKGROUND： The lipid peroxide, monoamine oxidase, and superoxide dismutase are the important biochemical pharmacological indexes to test the ability of eliminating the free radicals of organism. The lipid peroxide is also a quantitative index of senility oxidation.OBJECTIVE： To test the effect of taibai yangshen on resisting free radicals through testing the serum lipid peroxide, monoamine oxidase （MAO）,and superoxide dismutase （SOD） of the senile rats.DESIGN： A random control experiment.SETTING： Pharmacy Department of Shaanxi College of Traditional Chinese Medicine （TCM）.MATERIALS： The experiment was done in March 2002 in the pharmacological Experimental Base of Shaanxi College of TCM. The 40 healthy young rats and 40 old rats were selected. The water extract of ground taibai yangshen was concentrated to 1.5 g/mL. The ginseng decoction was concentrated to 0.3 g/mL.METHODS： The young and old rats were randomized into 8 groups：young normal control group, young ginseng group, young taibai yangshen big dosage group, young taibai yangshen small dosage group, old normal control group, old ginseng group, old taibai yangshen big dosage group, and old taibai yangshen small dosage group. Ten rats were in each group. Seven days for adaptation to the environment. Then the normal control group was gastro-perfused daily with the distilled water 20 g/kg, the ginseng the taibai yangshen big and small dosage groups were gastro-perfused daily spectively. Thirty-five days later, the rats were cut heads in the state of anesthesia. The serum was centrifuged. The sulfuration barbituric acid was adopted to test the concentration of malondialdehyde （MDA）, the ultraviolet absorption was adopted to test the activity of MAO, and the xanthine oxidase was adopted to test the activity of SOD.MAIN OUTCOME MEASURES： The concentration of MDA and the activity of MAO and SOD.groups and the taibai yangshen big and small dosage groups, the concentration of MDA and the activity of MAO were lower than that of the norm

关 键 词：痴呆 自由基 过氧化脂质类 单胺氧化酶 超氧化物歧化酶 

分 类 号：R229[医药卫生—中医基础理论]