慢性脑缺血大鼠海马CA_1区中的BDNF表达  

THE EXPRESSION OF BDNF IN HIPPOCAMPAL CA_1 AREA IN CHRONIC CEREBRAL ISCHEMIA RATS

作　　者：巴迎春[1] 王廷华[2] 潘兴华[3] 李明[1] 王金德[1] 李朗[1] 温淑仪[1] 

机构地区：[1]昆明医学院解剖学教研室,昆明650031 [2]昆明医学院神经科学研究所,昆明650031 [3]成都军区昆明总医院医学实验科,昆明650032

出　　处：《神经解剖学杂志》2005年第6期664-666,共3页Chinese Journal of Neuroanatomy

摘　　要：为了观察慢性脑缺血时海马CA1区中BDNF的表达变化,探讨缺血性脑损伤及修复机制。我们将Wistar大鼠双侧颈总动脉结扎制成慢性脑缺血动物模型。分为三组:正常对照组、慢性脑缺血30d和120d组。分别于术后30d和120d处死动物,行BDNF免疫组化染色,计数各组大鼠海马CA1区中的阳性神经元数。结果显示:(1)在对照组的海马CA1区可见BDNF较强的表达;(2)在慢性脑缺血30d时,海马CA1区BDNF的表达高于缺血120d组及对照组(P<0.05)。而120d时,海马CA1区BDNF的表达与对照组无显著性差异(P>0.05)。结果提示:(1)在正常大鼠海马CA1区有BDNF表达,能维持神经元的存活;(2)慢性脑缺血时,BDNF在海马CA1区的表达先升后降。To observe the expression of BDNF in hippocampal CA1 area in chronic cerebral ischemia rats, and explore the mechanisms of cerebral ischemia damage and recovery. The chronic cerehral ischemia model of Wislar rats were made by clipping douhle common carotid arteries. The rats were divided into three groups： control group, the groups of chromic cerehral ischemia for 30 and 120 d. The rals were sacrificed on the 30^th and 120^th d. then the BDNF immunohistochemical staining were performed. The positive neurons of hippocampal CA1 area were counted in each group. The results showed that： （ 1 ） The expression of BDNF in hippocampal CA1 area of control group were intense： （2） On the 30^th d after cerebral ischemia, the expression of BDNF in hippocampal CA1 area was ohviously increased than those of the 120^th d after cerebral ischemia and control groups. On the 120^th d after cerebral ischemia, the expression of BDNF in hippoeampal CA1 area showed no obvious difference with that of control group. The results suggest thai： （ 1 ） The expression of BDNF in hippocampal CA1 area in normal rats can maintain the neurons＇ survival; （2） After the chronic cerebral ischemia, the expression of BDNF in hippocampal CA1 area firstly increased,and subsequently declined.

关 键 词：脑缺血 BDNF 海马CA1区 

分 类 号：R743.3[医药卫生—神经病学与精神病学]