豚鼠上半规管切除对内耳的影响  

The Influence on the Inner Ear of the Guinea Pigs Following Excision of the Semicircularis Superior

作　　者：李永团[1] 迟晓艳[1] 董频[2] 蔡敏[2] 李晓艳[2] 王强[2] 王国良[2] 王文栋[2] 

机构地区：[1]青岛市市立医院耳鼻咽喉-头颈外科,266011 [2]上海交通大学附属第一人民医院耳鼻咽喉-头颈外科,上海200080

出　　处：《中国眼耳鼻喉科杂志》2005年第6期350-352,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

摘　　要：目的观察上半规管切除对豚鼠内耳功能的影响。方法 16只豚鼠随机分为4组,切除单侧上半规管,观察手术后动物行为及听性脑干反应,并于手术后7d 和28d 处死试验动物,硝酸银染色耳蜗基底膜铺片和石蜡切片了解内耳形态学变化。结果术后2～3d 内,豚鼠头向术侧倾斜,行走不稳,以后恢复;术后即刻 ABR 阈值升高,和术前相比差异有显著性,术后1、4wk 与术前相比差异无显著性;病理切片检查壶腹嵴毛细胞、耳蜗各阶形态和 Corti 器正常,切除部位有炎性细胞和纤维组织形成。手术前后基底膜铺片内外毛细胞计数统计学处理差异无显著性(P>0.05)。结论上半规管切除对豚鼠耳蜗及前庭感受器功能的影响不大。(中国眼耳鼻喉科杂志,2005,5:350～352)Purpose To observe the disturbance of the guinea pigs inner ear following the anterior semicircular canals transection and plugging. Methods Sixteen mixed-coloured guinea pigs with single anterior semicircular canal excision were used and divided randomly into 4 groups in our study. The auditory function was assessed by measuring the auditory brainstem response （ABR） thresholds. The animal＇s behavior and the inner ear morphological study were oberserved after the surgery. On 7 days, 28 days the animals were killed and the cochleas were stained with AgNO3 or processed to obtain section from plastic embedded ears. Results The guinea pigs＇ head inclined to the operative side and walked ansteadly two or three days after the surgery, then recovered. ABR showed that the operations had little effect on the ABR thresholds after a long time and there was significant difference between preoperation and the postoperation moment. Histologically, there was intraluminal fibrosis and inflammation surround surgical field. Most specimens showed normal cochlear anatomy and hair cell counts. The inner or out hair cell analysis showed no statistical diffrence before and after surgery. Conclusion The excision of the anterior semicircular canal is a safe procedure with little effect on the heating and vestibular function.

关 键 词：半规管手术 听力 毛细胞 

分 类 号：R764[医药卫生—耳鼻咽喉科]