侵染广西罗汉果的小西葫芦黄花叶病毒的鉴定及抗血清制备  

Identification of a Zucchini Yellow Mosaic Virus Isolate from Siraitia grosvenori(Swingle) C.Jeffrey in Guangxi Province and Preparation of Coat Protein Antiserum

作　　者：秦碧霞[1] 郑红英[2] 蔡健和[1] 陈炯[2] 刘志明[1] 陈剑平[2] 

机构地区：[1]广西农业科学院植物保护研究所,广西南宁530007 [2]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021

出　　处：《病毒学报》2005年第6期468-473,共6页Chinese Journal of Virology

基　　金：浙江省自然科学基金重点项目(ZA0207);国家自然科学基金项目(30200008);广西青年科学基金资助项目(桂科青0135006);浙江省重大招标项目(030823010);广西"新世纪十百千人才工程"项目(2001215);广西农科院科技发展基金项目(2003003Z)

摘　　要：在广西临桂罗汉果花叶畸形病株上获得了一个线状病毒分离物LGL-1,寄主范围、蚜传能力测定、病毒粒子形态和细胞病理特征研究表明,它是马铃薯Y病毒科成员。采用马铃薯Y病毒科特异性简并引物做PCR扩增,并测定了分离物LGL-1的基因组3′-末端序列,序列分析表明它是小西葫芦黄花叶病毒(ZYMV)。系统进化树分析揭示,世界范围内的ZYMV分离物主要可分为3大群体,分离物LGL-1为中国特有群体Group III成员。原核表达制备了分离物LGL-1的外壳蛋白抗血清,明确了ZYMV是引起广西罗汉果病害的主要病毒,并且比较了原核表达法和提纯病毒法制备的抗血清,在病样的间接ELISA法检测中结果的差异。A filamentous virus was found that causes rugose and mosaic symptoms in Siraitia grosvenorii （Swingle） C.Jeffrey in Yongfu county of Guangxi Province. Based on its biological, morphological and cy topathological characteristics, isolate LGL-1 was primarily identified as a member of family Potyviridae. Using the degenerated primer of family Potyviridae, the 3＇-terminal sequence of isolate LGL-1 was determined. BLAST search confirmed that it was an isolate of Zucchini yellow mosaic virus （ZYMV）. Phylogenetic analysis based on the coat protein nucleotide sequences of ZYMV isolates confirmed the existence of three main groups. LGL-1 belongs to the newly reported Group III which is only found in China so far. A polyclonal antiserum of isolate LGL 1 was raised against the expected coat protein. Indirect ELISA result suggested that ZYMV was the main potyvirus harmful to the production of Luohanguo plants in Guangxi Province. The differences between this antiserum and the antiserum prepared by using purified viral particles in ELISA were also discussed.

关 键 词：生物学鉴定 系统进化树 小西葫芦黄花叶病毒 原核表达 间接ELISA 

分 类 号：S432.41[农业科学—植物病理学]