D6S2418在中国(汉族)、泰国和德国人群中的遗传多态性  

Research on the Genetic Polymorphisms of D6S2418 Locus in Chinese Han Ethnic, Thai and Germany Populations

作　　者：刘志勇[1] 陈国弟[1] 任广田[2] 余琳[1] 韩军鸽[1] 王晶[1] 廖淼[1] 梁伟波[1] 张林[1] 

机构地区：[1]四川大学华西基础与法医学院法医物证教研室,四川成都610041 [2]深圳市公安局盐田分局,广东深圳518082

出　　处：《法医学杂志》2005年第4期283-285,共3页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30171033)

摘　　要：目的获得D6S2418基因座的群体遗传学数据,分析其基因频率在不同群体间的分布情况是否存在差异,并分析其在法医学中的应用价值。方法采用PCR、非变性聚丙烯酰胺凝胶电泳(PAGE)及银染技术分析中国成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群中D6S2418基因座的遗传多态性,获得三个群体D6S2418基因座的群体遗传学数据。结果从300份分别采自成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群三个群体的无血缘关系个体的静脉血,共发现9个等位基因,观测到31种基因型。观测杂和度为64%～81%,个人识别机率为84.2%～93.5%,经统计学检验,基因型的频率分布符合Hardy-Weinberg平衡定律。等位基因频率的分布在三个群体间有显著差异。结论D6S2418基因座的个人识别能力高,在法医学个人识别和亲子鉴定应用中有较高价值。Objective To obtain the genetic data on STR locus D6S2418,and analyzed its genetic polymorphism in 3 different races （Han,Thai and Germany）. Methods The blood samples from 300 unrelated individuals were analyzed by PCR,PAG electrophoresis and silver staining. Results 9 alleles and 31 genotypes were observed.The observed heterozygosity and the discrimination power were 64%-81% and 84.2%-93.5%,respectively.The genotype distributions of D6S2418 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious. Conclusion The methods and exclusion chances of this locus founded are easy to perform and are desirable for forensic analysis sensitive. The discrimination power and application.

关 键 词：短串联重复序列 PCR D6S2418 遗传多态性 

分 类 号：R394[医药卫生—医学遗传学] Q347[医药卫生—基础医学]