斜卧青霉P6木素过氧化物酶的褐煤降解活性及cDNA克隆  

cDNA Clone and Lignite Degradation Ability of Lignin Peroxidase from Penicillium decumbens P6

作　　者：杨金水[1] 袁红莉[1] 陈文新[1] 

机构地区：[1]中国农业大学生物学院

出　　处：《北京大学学报（自然科学版）》2005年第6期833-839,共7页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金(30370040);国家高技术研究发展计划(2003AA241170)资助项目

摘　　要：对纯化得到的斜卧青霉P6分泌的木素过氧化物酶的褐煤降解活性进行了研究,发现纯酶的褐煤降解活性依赖于H2O2的激活。降解后产物中小分子黄腐酸含量显著提高,褐煤及降解产物的经验分子式分别为C100H86·5O36·5N2·09和C100H103·9O48·6N11·4,降解产物比原褐煤具有更高的H、O、N含量,而C含量明显下降。并且降解产物可提高豌豆种子的发芽率并明显缩短发芽时间。根据N末端氨基酸序列设计特异性引物,利用RT-PCR方法扩增得到一条约2000bp的cDNA片段,序列测定的大小为1956bp,通过序列比对,与已报道的Piloderma croceum菌的mnp2基因具有45%的同源性,具有过氧化氢酶的保守氨基酸残基。The present study was undertaken to investigate the degradation ability and biological activity of purification lignin peroxidase （LIP） from Penicillium decumbens Pr. P6 LiP had very good degradation ability on lignite, but its activity must depend on H2 02 aetiviation. After degradation, the contents of humie acid and fulvie acid increased distinctly, especially the content of fulvie acid. Results of elemental analysis indicated that the biodegraded products had higher H,O and N levels than the original lignite, although the carbon content was distinctly lower. The experimental molecular formula for lignite and its biodegraded products were C100 H86.5 036.5 N2.09 and C100 H103.9 O45.6 N11.4 separately. The products of degradation could increase the germination rate and the speed of germination of pea. Furthermore, the authors designed two special primers according to N terminal amino acid sequences and got a 1 956 bp cDNA fragment. Sequence comparison demonstrated that it had 45 % homology with Piloderma croceum mnp2 gene and had conservative amino acids of catalase.

关 键 词：褐煤 斜卧青霉 木素过氧化物酶 克隆 

分 类 号：Q78[生物学—分子生物学]