葛根素对急性缺血脑神经细胞损伤的保护及对HSP_(70)蛋白表达的干预作用  

Protection of puerarin and its effect on the expression of HSP_(70) after acute local ischemia brain injury in rat

作　　者：杨嘉珍[1] 潘洪平[1] 李吕力[1] 莫祥兰[1] 黄进[1] 黄振录[1] 叶劲[1] 焦伟[1] 董艳玲[1] 

机构地区：[1]广西壮族自治区人民医院,南宁530021

出　　处：《广西医学》2005年第11期1721-1723,共3页Guangxi Medical Journal

基　　金：广西壮族自治区卫生厅医药卫生科研课题(Z2001072)

摘　　要：目的探讨葛根素对大鼠急性脑缺血损伤的保护作用和对HSP70表达的影响及其作用机制。方法采用闭夹大脑中动脉造成局部脑缺血模型,对实验标本作HE染色及用免疫组化SP法作HSP70染色,观察其组织病理学改变及HSP70表达情况。结果葛根素干预组死亡神经元数明显少于单纯缺血组,脑水肿程度较轻,而HSP70表达强度显著强于单纯缺血组。葛根素干预暴露因素与神经细胞死亡呈显著负相关(P<0.01)。结论葛根素对大鼠急性脑缺血损伤有保护作用,其作用机制与上调HSP70的表达有关。Objective To study the protection of puerarin and is effect on the expression of HSP70 after acute local ischemia brain injury in rat,as well as the mechanism of its fimcfions.Methods Rat model of acute local cerebral ischemia was made by ligated middle cerebral artery. The histopathological damage was assessed and the HSP70 expression was detected by histochemistry in SP method. Results The number of death neurom were significantly less and the expression of HSP70 was strongly higher in puemrin treated rats than those with ischemia rats. Conclusions Puerarin can prevent the neuron damage after acute ischemia brain injury in rats. The mechanism of its function maybe relate to improving the level of HSP70 expression.

关 键 词：葛根素 HSP70 脑急性缺血 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]