抗人脑胶质瘤单克隆抗体SZ－39轻链可变区基因的克隆与序列分析  

cDNA cloning of variable region of monoclonal antibody SZ-39 specific for human brain glioma

作　　者：沈树泉[1] 周筱梅[1] 黄强[1] 周丽英[1] 杜子威[1] 

机构地区：[1]苏州医学院附二院脑外科,上海市肿瘤研究所生化室

出　　处：《中华神经外科杂志》1996年第2期72-74,共3页Chinese Journal of Neurosurgery

基　　金："八五"核工业部攻关课题

摘　　要：从抗人脑胶质瘤单克隆抗体杂交瘤细胞中抽提总ＲＮＡ，并以Ｏｌｉｇｏ（ｄＴ）柱纯化ｍＲＮＡ，经逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）直接扩增出３１８ｂｐ的轻链可变区（ＶＬ）ｃＤＮＡ片断，经核苷酸序列分析研究，证实已获得了ＳＺ－３９单抗轻链可变区基因。这为今后进一步制备基因工程抗体打下基础。We both have extracted total RNA and purified mRNA by(dT)cellulose in the hybridoma cell of anti-human brain glioma monoclonal antibody and have designed a set of oligonucleotide primers to amplify the cDNA of mouse immunoglobulin light chain variable-region gene by polymerase chain reaction.The primers incorporate restriction sites that allow the cDNA of the variable domain to be force-cloned for sequencing and expression.The result shows that the length of light chain variable-region gene of this antibody is 318 base pairs. This study based for antibody production with genetic engineering.

关 键 词：单克隆抗体 可变区基因 胶质瘤 脑肿瘤 

分 类 号：R739.410.2[医药卫生—肿瘤]