抗人脑胶质瘤单克隆抗体SZ—39可变区基因特征的初步分析  

Characierization of the Variable Regions of Monoclonal Antibody SZ—39 Specific for Human Brain Glioma

作　　者：王爱东[1] 黄强[1] 许志元 沈树泉[1] 杜子威[1] 

机构地区：[1]苏州医学院附属第二医院脑肿瘤研究室

出　　处：《苏州医学院学报》1996年第2期223-225,399,共3页Acta Academiae Medicinae Suzhou

基　　金：中国核工业总公司基金题

摘　　要：应用逆转录聚合酶链式反应(RT—PCR)技术扩增出单克隆抗体SZ—39可变区基因,以测序证实重链可变区基因(VH)长348bp,编码116个氨基酸,轻链可变区基因(VL)长318bp,编码106个氨基酸。同源性比较显示它们与小鼠免疫球蛋白可变区基因同源性最高,分别达88%和65%。SZ—39单抗重链隶属小鼠Ig重链第Ⅱc亚组,VD.J_(a2)基因重排;轻链隶属小鼠Ig Kappa轻链第Ⅱ亚组,JK_2基因重排。The genes of variable region of monoclonal antibody SZ—39 were cloned by reverse transeriptase-PCR method. Nucleotide sequences analysis showed that SZ—39VH is a nudeotide with 348 base pairs, encoding 116 amino acids, and the light one is composed of 318 base paris, encoding 106 amino acids. Structual analysis of the amino acid sequences shows that heavy chain variable domain of SZ—39 McAb belongs to the mouse Ig heavy subgroup Ⅱc and V_H—D—J_(H2) rearrangement variable region of light chain belongs to sub Ⅱ group of mouse V_K family V—J_(K2) assembly.

关 键 词：单克隆抗体 可变区基因 胶质瘤 脑肿瘤 

分 类 号：R739.410.2[医药卫生—肿瘤]