人卵巢癌顺铂耐药细胞中核糖体蛋白基因的表达谱分析  

Expression profile of ribosomal protein genes in cisplatin resistant COC_1/DDP cells of human ovarian carcinoma

作　　者：曹漫明[1] 张积仁[1] 周媛[1] 孙海[1] 李工[1] 

机构地区：[1]南方医科大学(原第一军医大学)附属珠江医院肿瘤中心,广州510282

出　　处：《肿瘤》2005年第6期555-558,共4页Tumor

基　　金：国家自然科学基金(编号:39870813)

摘　　要：目的研究核糖体蛋白基因在人卵巢癌顺铂耐药细胞中的表达。方法以顺铂为诱导剂,采用中等剂量、间歇作用法建立人卵巢癌顺铂耐药细胞系COC_1／DDP,然后以其亲本细胞COC_1为对照,应用cDNA微阵列检测COC_1／DDP细胞的核糖体蛋白基因表达。结果与COC_1细胞相比,COC_1／DDP细胞对顺铂有6．2倍耐药性,细胞倍增时间延长了8．9h,S期细胞减少了(27．4±2．13)％,而G_1和G_2期细胞分别增加了(19．6±3．71)％和(8．3±1．95)％。在143个表达水平发生改变的基因中,有12个核糖体蛋白基因表达下调,多个凋亡抑制基因表达上调,凋亡诱导基因CASP2表达下调。结论 COC_1／DDP细胞对顺铂具有耐药性,耐药性的产生可能与部分核糖体蛋白基因的表达下调有关。Objective To study the expression of ribosomal protein genes in cisplatin resistant COC1/DDP human ovarian carcinoma cells. Methods Cisplatin resistant COC1/DDP cells were induced by moderate concentration and intermittent action with cisplatin, the differential expressions of ribosomal protein genes between the parental COC1 and COC1/DDP cells were determined with cDNA microarray. Results COC1/DDP cells were resistant to DDP with its IC50 of DDP was 6.2 times higher than that of COC1 ,and it had a prolonged doubling time of 8.9 hours, the number of cells in S-phase was significantly decreased （27.4 ± 2. 13） % while those in G1 and G2 phase increased （ 19. 6 ± 3.71 ） % and （8.3 ± 1.95） %, respectively. Compared to COC1 , 143 genes were differentially expressed significantly in COC1/DDP cells, among which 12 ribosomal protein genes were down-regulated significantly, many anti-apoptotic genes were up-regulated and apoptotic gene CASP2 was down-regulated. Conclusion COC1/DDP is cisplatin resistant, and down-regulation of ribosomal protein genes might be involved in cisplatin resistance development.

关 键 词：卵巢肿瘤 顺铂抗药性 核糖体蛋白质类 寡核苷酸类排列序列分析 基因表达 肿瘤细胞 培养的 

分 类 号：R737.31[医药卫生—肿瘤] R73-36[医药卫生—临床医学]