子宫内膜癌组织中雌激素受体相关受体亚型的表达及其意义  

Expression of estrogen receptor-related receptor isoforms in endometrial carcinoma tissues and its clinical significance

作　　者：高敏[1] 魏丽惠[1] 孙蓬明[1] 赵丹[1] 王建六[1] 王志启[1] 赵超[1] 

机构地区：[1]北京大学人民医院妇科,100044

出　　处：《中华妇产科杂志》2005年第11期756-760,共5页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目(30371477)

摘　　要：目的探讨雌激素受体相关受体(ERR)亚型在子宫内膜癌组织中的表达及其临床意义。方法选取46例子宫内膜癌及24例正常子宫内膜新鲜组织,采用RT-PCR技术和免疫组化法检测ERR亚型α、β、γ在组织中的表达,并根据雌激素受体(ER)α的表达情况将其分为ERα(+)和ERα(-)者。结果ERRα、β、γ的mRNA和蛋白表达之间均呈明显正相关关系(r=0.462,P=0.009;r=0.689,P=0.016;r=0.673,P=0.001)。内膜癌组织ERα(+)者中,ERRαmRNA阳性表达率及表达水平(分别为42%,0.24±0.18)均明显低于正常内膜组织ERα(+)者(分别为78%,0.42±0.23;P=0.019,P=0.021);内膜癌组织中ERRβmRNA阳性表达率及表达水平(分别为23%,0.21±0.16)与正常内膜组织(分别为22%,0.27±0.15)相比,差异无统计学意义(P>0.05);ERRγmRNA在内膜癌及正常内膜组织中均呈现高表达,ERα(+)的内膜癌组织中ERRγmRNA的表达水平(0.93±0.24)明显高于正常内膜组织(0.72±0.21,P=0.023)。ERRαmRNA表达阳性的内膜癌患者中,手术病理分期为Ⅰ期患者所占百分比(30%)明显低于ERRαmRNA表达阴性者(70%,P=0.017),但其Ⅱ～Ⅳ期患者及深肌层浸润患者所占百分比(分别为70%和78%)均明显高于ERRαmRNA表达阴性者(分别为30%和43%;P=0.017,P=0.033);内膜癌组织中ERRβmRNA的表达与手术病理分期、病理分级、肌层浸润及淋巴结转移无关(P>0.05);ERRγmRNA表达阳性的内膜癌患者中,淋巴结有转移患者所占百分比(18%)明显低于ERRγmRNA表达阴性者(58%,P=0.021),但其与手术病理分期、病理分级及肌层浸润程度无关(P>0.05)。结论ERR亚型可能是ER以外与子宫内膜癌发生关系密切的又一受体。ERRα可能是子宫内膜癌预后不良的指标,而ERRγ高表达可能提示预后良好。Objective To investigate the role of estrogen receptor-related receptor （ ERR）, a subfamily of orphan receptors, in the tumorigenesis of endometrial carcinoma and to evaluate the clinical significance. Methods A total of 46 cases of endometrial carcinoma tissues and 24 cases of normal endometrium tissues were collected. Semi-quantitive reverse transcription polymerase chain reaction and immunohistochemistry were used to measure the expressions of ERR. All samples were classified into two groups according to ERα status. Results It was positively correlated between the ERR （ α, β, γ） mRNA and protein expression （r = 0. 462, P = 0. 009; r = 0. 689, P = 0. 016 and r = 0. 673, P = 0. 001 ）. The expression rate and level of ERRα mRNA in ERα-positive endometrial carcinoma （42% ,0.24±0. 18）were lower than in normal endometrium（78% ,0. 42 ±0. 23 ;P =0. 019,P =0. 021 ）. No significant difference was observed in the expression of ERRβ mRNA between endometrial carcinoma （23% ,0. 21 ± 0. 16）and normal endometrium （22% ,0. 27 ± 0. 15 ;P 〉 0. 05 ）. In ERα-positive endometrial carcinoma, the expression level of ERRγ, mRNA （ 0. 93 ± 0. 24 ） was higher than normal endometrium （ 0. 72 ± 0. 21 ; P = 0. 023 ）. The numbers of patients of Federal International Gynecological Oncology （FIGO） stage Ⅱ-Ⅳ and of deep myometrial invasion in ERRα mRNA positive endometrial carcinoma group were more than those in ERRα mRNA negative group （P =0. 017 and P=0. 033）. ERRγ mRNA positive patients had a lower occurrence of lymph metastases than negative patients （ P = 0. 021 ）. Conclusions ERR may be closely related with the tumorigenesis of endometrial carcinoma, similar to ER. ERRα may serve as a biomarker of poor prognosis and ERRγ, as a favorable biomarker in endometrial carcinoma.

关 键 词：子宫内膜肿瘤 受体 雌激素 

分 类 号：R737.33[医药卫生—肿瘤] R737.9[医药卫生—临床医学]